Kikuchi Y, Irie M, Kasahara T, Sawada J, Terao T
Department of Microbiology, Hoshi College of Pharmacy, Tokyo, Japan.
FEBS Lett. 1993 Feb 8;317(1-2):22-6. doi: 10.1016/0014-5793(93)81483-g.
The effects of cytokines and heavy metals on the expression and localization of metallothioneins (MTs) within U373MG astrocytoma cells were analyzed by using indirect immunofluorescence using a monoclonal anti-MT antibody (MT45). IL-1, CdCl2 (50 microM) or ZnCl2 (500 microM) remarkably augmented intracellular MT levels, whereas IL-6 or 10 microM of ZnCl2 showed no inducing activity. From 24 to 48 h after the addition of CdCl2 or IL-1, immunoreactive MTs were found in the cytoplasm and the nucleus. After 72 h, immunoreactive MTs accumulated in a granular form near the cell surfaces in the presence of CdCl2 (50 microM) or IL-1 plus ZnCl2 (10 microM). However, this accumulation was not observed when only IL-1 was added. Thus, Zn2+ facilitated the appearance of the granular form of immunoreactive MTs at a concentration where they do not induce MTs by themselves.
通过使用单克隆抗金属硫蛋白抗体（MT45）进行间接免疫荧光分析，研究了细胞因子和重金属对U373MG星形细胞瘤细胞内金属硫蛋白（MTs）表达和定位的影响。白细胞介素-1、氯化镉（50微摩尔）或氯化锌（500微摩尔）显著提高细胞内MT水平，而白细胞介素-6或10微摩尔的氯化锌则无诱导活性。添加氯化镉或白细胞介素-1后24至48小时，在细胞质和细胞核中发现免疫反应性MTs。72小时后，在存在50微摩尔氯化镉或白细胞介素-1加10微摩尔氯化锌的情况下，免疫反应性MTs以颗粒形式聚集在细胞表面附近。然而，仅添加白细胞介素-1时未观察到这种聚集。因此，锌离子在其自身不诱导MTs的浓度下促进了免疫反应性MTs颗粒形式的出现。
