Corrier D E, Adams L G
Am J Vet Res. 1977 Feb;38(2):217-22.
Ultrastructural renal lesions were studied in 20 goats given one intramuscular injection of a lethal dose of imidocarb diproprionate (6.75 mg/kg). The lesions were limited to proximal convoluted tubular epithelial cells and at postinjection (PI) hour 3 included: plasma membrane disruption, smooth endoplasmic reticulum proliferation and dilatation, swelling and increased electron density of mitochondria, and cytoplasmic ground substance rarefaction. At PI hour 12, disruption of plasma membranes, with fragmentation of the microvilli, loss of junctional complexes, and cellular detachments from in tact basement membranes were observed in many tubular epithelial cells. Mitochondrial configurational alterations became more prominent at 6 and 12 hours, with the formation of large membrane-bounded structures containing fragmented cristae, clear vacuoles, and amorphous matrical material. The intramitochondrial foci of electron-dense amorphous flocculent material, which was first observed at 6 hours and increased in electron density and in number by 12 and 24 hours, were probably comprised of denatured, clumped matrical protein. The 2nd type of intramitochondrial and cytoplasmic electron-dense deposit was observed PI hour 24. The basement membranes remained intact in most proximal tubules to support regenerating epithelial cells on PI day 4 and thereafter.
对20只山羊进行研究，每只山羊肌肉注射致死剂量的双丙酸咪唑苯脲（6.75毫克/千克），观察其超微结构肾损伤情况。损伤仅限于近端曲管上皮细胞，注射后3小时可见：质膜破坏、滑面内质网增生和扩张、线粒体肿胀及电子密度增加、细胞质基质稀疏。注射后12小时，许多肾小管上皮细胞可见质膜破坏，微绒毛断裂，连接复合体丧失，细胞从完整的基底膜脱离。线粒体形态改变在6小时和12小时时更为明显，形成了含有破碎嵴、清亮空泡和无定形基质物质的大的膜包被结构。线粒体内部电子致密的无定形絮状物质灶最早在6小时时观察到，到12小时和24小时时电子密度和数量增加，可能由变性、聚集的基质蛋白组成。注射后24小时观察到第二种线粒体和细胞质电子致密沉积物。在注射后第4天及之后，大多数近端小管的基底膜保持完整，以支持再生的上皮细胞。
