Zhou B K, Boissy R E, Pifko-Hirst S, Moran D J, Orlow S J
Ronald O. Perelman Department of Dermatology, New York University Medical Center, New York.
J Invest Dermatol. 1993 Feb;100(2):110-4. doi: 10.1111/1523-1747.ep12462775.
Coated vesicles play a critical role in the process of melanogenesis. Antisera raised against a coated vesicle fraction from mouse melanoma cells recognize two major glycoprotein antigens, band I (47-55 kd) and band II (90-120 kd). We demonstrate that band II is lysosome-associated membrane protein 1 (LAMP-1) by the following criteria: 1) the molecular weight and abundance of LAMP-1 varies among tissues but is always identical to that of band II; 2) band II and LAMP-1 co-migrate in sucrose gradient sedimentation studies; 3) immunodepletion of cell extracts with antivesicle serum removes all LAMP-1; and 4) intact organelles immunoisolated with antivesicle serum contain band II and LAMP-1. Our results further confirm the long-suspected relationship between melanosomes and the lysosomal lineage of organelles.
被膜小泡在黑色素生成过程中起关键作用。用从小鼠黑色素瘤细胞的被膜小泡组分制备的抗血清可识别两种主要的糖蛋白抗原，即I带（47 - 55千道尔顿）和II带（90 - 120千道尔顿）。我们通过以下标准证明II带是溶酶体相关膜蛋白1（LAMP - 1）：1）LAMP - 1的分子量和丰度在不同组织中有所变化，但总是与II带相同；2）在蔗糖梯度沉降研究中，II带和LAMP - 1共同迁移；3）用抗小泡血清对细胞提取物进行免疫去除可去除所有LAMP - 1；4）用抗小泡血清免疫分离的完整细胞器含有II带和LAMP - 1。我们的结果进一步证实了长期以来人们所怀疑的黑素体与溶酶体细胞器谱系之间的关系。
