de Haan P, Kormelink R, de Oliveira Resende R, van Poelwijk F, Peters D, Goldbach R
Department of Virology, Agricultural University, Wageningen, The Netherlands.
J Gen Virol. 1991 Sep;72 ( Pt 9):2207-16. doi: 10.1099/0022-1317-72-9-2207.
The complete nucleotide sequence of the large (L) genome segment of tomato spotted wilt virus (TSWV) has been determined. The RNA is 8897 nucleotides long and contains complementary 3' and 5' ends, comprising 62 nucleotides at the 5' end and 66 nucleotides at the 3' end. The RNA is of negative polarity, with one large open reading frame (ORF) located on the viral complementary strand. This ORF corresponds to a primary translation product of 2875 amino acids in length, with a predicted Mr of 331,500. Comparison with the polymerase proteins of other negative-strand viruses indicates that this protein most likely represents the viral polymerase. The genetic organization of TSWV L RNA is similar to that of the L RNA segments of Bunyamwera and Hantaan viruses, animal-infecting representatives of the Bunyaviridae.
番茄斑萎病毒（TSWV）大（L）基因组片段的完整核苷酸序列已被确定。该RNA长8897个核苷酸，含有互补的3'和5'末端，5'端有62个核苷酸，3'端有66个核苷酸。该RNA为负链极性，在病毒互补链上有一个大的开放阅读框（ORF）。这个ORF对应一个长度为2875个氨基酸的初级翻译产物，预测分子量为331,500。与其他负链病毒的聚合酶蛋白比较表明，该蛋白很可能代表病毒聚合酶。TSWV L RNA的基因组织与布尼亚病毒科感染动物的代表病毒——布尼亚姆韦拉病毒和汉坦病毒的L RNA片段相似。
