Nakajima M, Hayakawa T, Nakamura I, Suzuki M
Plantech Research Institute, MKC Research Center, Yokohama, Japan.
J Gen Virol. 1993 Feb;74 ( Pt 2):319-22. doi: 10.1099/0022-1317-74-2-319.
Transgenic tobacco expressing the coat protein (CP) of cucumber mosaic virus strain O (CMV-O) showed a significant level of protection against CMV strains O and Y. When inoculum concentrations were increased, the transformants showed a stronger level of protection against CMV-O than against CMV-Y. The substitution of an amino acid residue between CMV-O and CMV-Y, which is presumed to change the conformation of CP, may reflect the difference in susceptibility to these viruses. Furthermore the transgenic tobacco plants showed a significant level of protection against chrysanthemum mild mottle virus, a member of the cucumovirus group but with no serological relationship to CMV.
表达黄瓜花叶病毒O株（CMV - O）外壳蛋白（CP）的转基因烟草对CMV的O株和Y株表现出显著的保护水平。当接种物浓度增加时，转化体对CMV - O的保护水平比对CMV - Y的保护水平更强。CMV - O和CMV - Y之间一个氨基酸残基的替换，推测会改变CP的构象，这可能反映了对这些病毒易感性的差异。此外，转基因烟草植株对菊花轻斑驳病毒表现出显著的保护水平，菊花轻斑驳病毒是黄瓜花叶病毒组的成员，但与CMV无血清学关系。
