胰腺前原脂酶及其与胰腺脂肪酶复合物的结晶
Crystallization of pancreatic procolipase and of its complex with pancreatic lipase.
作者信息
van Tilbeurgh H, Gargouri Y, Dezan C, Egloff M P, Nésa M P, Ruganie N, Sarda L, Verger R, Cambillau C
机构信息
Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, URA1296-CNRS, Faculté de Médecine Secteur-Nord, Marseille, France.
出版信息
J Mol Biol. 1993 Jan 20;229(2):552-4. doi: 10.1006/jmbi.1993.1054.
The human pancreatic lipase-porcine procolipase complex has been crystallized in space group P3(2)21 (a = b = 80.3 A and c = 251 A) from a solution containing polyethylene glycol, NaCl and beta-octyl glucoside. The crystals diffract to 2.6 A on a synchrotron beam. The complex in the presence of bile salts and phospholipids crystallizes in a tetragonal space group P4(2)2(1)2 (a = b = 133.4 A, c = 92.6 A). Crystals of procolipase alone were obtained under slightly different experimental conditions (space group I432, a = b = c = 164.3 A).
人胰脂肪酶-猪前原脂酶复合物已从含有聚乙二醇、氯化钠和β-辛基葡糖苷的溶液中，在空间群P3(2)21（a = b = 80.3 Å，c = 251 Å）中结晶。这些晶体在同步加速器光束上衍射至2.6 Å。在胆汁盐和磷脂存在下的该复合物在四方空间群P4(2)2(1)2（a = b = 133.4 Å，c = 92.6 Å）中结晶。单独的前原脂酶晶体是在略有不同的实验条件下获得的（空间群I432，a = b = c = 164.3 Å）。
Zotero 插件