Reiken S R, Briedis D M
Department of Chemical Engineering, Michigan State University, East Lansing 48824-1226.
Leuk Res. 1993 Feb;17(2):121-8. doi: 10.1016/0145-2126(93)90056-q.
This paper describes the use of an immobilized enzyme reactor in the study of the in vitro effects of lysine deprivation on leukemic blood. L-lysine alpha-oxidase is immobilized in a single hollow fiber reactor to remove lysine from the blood of sheep infected by BLV. The treatment relies on the higher sensitivity of leukemic cells to nutrient depletion than that of normal cells. A population balance model is used to describe the changes in the leukocyte proliferative capacity after treatment. Additionally, preliminary data from in vitro tests with human blood demonstrate the potential of L-lysine alpha-oxidase and the enzymatic reactor in treating leukemia.
本文描述了固定化酶反应器在研究赖氨酸缺乏对白血病血液的体外影响中的应用。L-赖氨酸α-氧化酶固定在单个中空纤维反应器中，以去除感染BLV的绵羊血液中的赖氨酸。该治疗方法基于白血病细胞对营养物质耗竭的敏感性高于正常细胞。使用群体平衡模型来描述治疗后白细胞增殖能力的变化。此外，来自人体血液体外试验的初步数据证明了L-赖氨酸α-氧化酶和酶反应器在治疗白血病方面的潜力。
