Lukasheva E V, Rubtsova M Iu, Kovba G V, Berezov T T, Egorov A M
Dpt. of Biochemistry, Russian Peoples' Friendship University, Moscow, Russia.
Vopr Med Khim. 1997 Nov-Dec;43(6):566-75.
The goal of the present study was the development of the optimal method of co-immobilization of two enzymes: L-lysine alpha-oxidase from Trichoderma sp. and horseradish peroxidase. Commercial nitrocellulose, nylon and N+ nylon membranes were used as carriers. The immobilization was carried out either by absorbtion or by covalent binding with aldehyde groups. The aldehyde groups were attached to the surface of the carriers by UV-irradiation of membranes in the presence of p-azidotetrafluorobenzaldehyde. The optimal concentrations of reagents, enzymes and reaction conditions were found. The membranes with the co-immobilised L-lysine alpha-oxidase and peroxidase were shown to be useful for the determination of L-lysine concentrations.
来自木霉属的L-赖氨酸α-氧化酶和辣根过氧化物酶。使用商用硝酸纤维素、尼龙和N+尼龙膜作为载体。固定化通过吸附或与醛基共价结合进行。醛基通过在对叠氮四氟苯甲醛存在下对膜进行紫外线照射而附着在载体表面。找到了试剂、酶的最佳浓度和反应条件。结果表明，共固定化L-赖氨酸α-氧化酶和过氧化物酶的膜可用于测定L-赖氨酸浓度。
