Gilchrist D P, Darlington C L, Smith P F
Department of Psychology, University of Otago, Dunedin, New Zealand.
Pharmacol Biochem Behav. 1993 Jan;44(1):99-105. doi: 10.1016/0091-3057(93)90285-2.
The aim of the present study was to determine if the calcium channel antagonist flunarizine would affect the time course of vestibular compensation for unilateral labyrinthectomy (UL) in guinea pigs. Animals received either a single IP injection of flunarizine 1 h pre-UL or a series of IP injections every 6 h for 24 h post-UL, starting at 6 h post-UL. Flunarizine was dissolved in 50-100% DMSO or suspended in 10% Tween-80 and administered at a dose of 10 mg/kg in the pre-UL condition and 10 or 20 mg/kg in the post-UL condition. All injections were 1 ml/kg in volume. Spontaneous nystagmus (SN), yaw head tilt (YHT), and roll head tilt (RHT) were measured using video analysis. When dissolved in DMSO and administered 1 h pre-UL, 10 mg/kg flunarizine had a small but significant effect on the rate of RHT compensation; otherwise, flunarizine had no significant effects on SN, YHT, or RHT when dissolved in DMSO. When suspended in Tween-80, 10 mg/kg flunarizine pre-UL resulted in a significant decrease in SN frequency and YHT relative to the control group, although the magnitude of the differences was small. When 20 mg/kg was given post-UL, both SN and YHT showed a small but significant change in the rate of compensation. No significant differences in RHT were observed. These results demonstrate that IP administration of flunarizine at a dose of 10-20 mg/kg IP has little effect on vestibular compensation compared to the effects obtained with low IM doses (0.8 mg/kg) of verapamil given 1 h pre-UL.
本研究的目的是确定钙通道拮抗剂氟桂利嗪是否会影响豚鼠单侧迷路切除术后前庭代偿的时间进程。动物在单侧迷路切除术前1小时接受一次腹腔注射氟桂利嗪，或在单侧迷路切除术后6小时开始，每6小时进行一系列腹腔注射，持续24小时。氟桂利嗪溶解于50 - 100%二甲基亚砜（DMSO）或悬浮于10%吐温80中，在术前条件下以10 mg/kg的剂量给药，术后条件下以10或20 mg/kg的剂量给药。所有注射体积均为1 ml/kg。使用视频分析测量自发性眼球震颤（SN）、偏航性头部倾斜（YHT）和翻滚性头部倾斜（RHT）。当溶解于DMSO并在术前1小时给药时，10 mg/kg氟桂利嗪对RHT代偿速率有微小但显著的影响；否则，当溶解于DMSO时，氟桂利嗪对SN、YHT或RHT无显著影响。当悬浮于吐温80中时，术前10 mg/kg氟桂利嗪导致SN频率和YHT相对于对照组显著降低，尽管差异幅度较小。当术后给予20 mg/kg时，SN和YHT在代偿速率上均显示出微小但显著的变化。未观察到RHT有显著差异。这些结果表明，与术前1小时给予低剂量（0.8 mg/kg）静脉注射维拉帕米相比，腹腔注射10 - 20 mg/kg氟桂利嗪对前庭代偿几乎没有影响。
