用于检测小鼠肿瘤中转化基因的叙利亚仓鼠胚胎细胞系：检测与X射线相关的小鼠肿瘤中的转化基因。

Sasai H, Higashi T, Nakamori S, Miyoshi J, Suzuki F, Nomura T, Kakunaga T

Department of Oncogene Research, Osaka University, Japan.

Br J Cancer. 1993 Feb;67(2):262-7. doi: 10.1038/bjc.1993.50.

The Syrian hamster embryo cell lines, SHOK and MC-1, were used as recipient cells for DNA transfection assay to detect transforming genes in experimental mouse tumours. A mouse repeat sequence was utilised to check whether each transformed focus included mouse genomic DNA in the Hamster background. We investigated five mouse tumours that are related to X-ray radiation, and detected activated c-K-ras, c-mos, and c-cot oncogenes which induced foci of hamster cells. These results show that SHOK and MC-1 cells have unique properties for detecting transforming genes in experimental mouse tumours.

叙利亚仓鼠胚胎细胞系SHOK和MC-1被用作DNA转染试验的受体细胞，以检测实验性小鼠肿瘤中的转化基因。利用一个小鼠重复序列来检查在仓鼠背景下每个转化灶是否包含小鼠基因组DNA。我们研究了五种与X射线辐射相关的小鼠肿瘤，并检测到激活的c-K-ras、c-mos和c-cot癌基因，这些基因诱导了仓鼠细胞灶。这些结果表明，SHOK和MC-1细胞在检测实验性小鼠肿瘤中的转化基因方面具有独特的特性。