van Verseveld H W, Meijer E M, Stouthamer A H
Arch Microbiol. 1977 Feb 4;112(1):17-23. doi: 10.1007/BF00446649.
P/2e ratios were calculated from anaerobic chemostat cultures of Paracoccus denitrificans with nitrogenous oxides as electron acceptor. P/2e ratios were calculated, using the YmaxATP values determined for aerobic cultures. When succinate was the carbon and energy source the average P/2e values of the sulphate- and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. With gluconate as carbon and energy source and average P/2e values of the gluconate-limited with nitrate as electron acceptor and nitrate limited cultures were 0.9 and 1.1, respectively. leads to H+/O ratios measured in cells obtained from sulphate-, succinate, nitrite-, gluconate- and nitrate-limited cultures yielded respective average values of 3.4, 4.5, 3.5, 4.8 and 6.2 for endogenous substrates. From our data we conclude that sulphate- and nitrite- limitation causes the loss of site I phosphorylation. Nitrite has no influence on the maximum growth yield on ATP. We propose that metabolism in heterotrophically grown cells of Paracoccus dentrificans is regulated on the level of phosphorylation in the site I region of the electron transport chain.
以含氮氧化物作为电子受体，从反硝化副球菌的厌氧恒化器培养物中计算P/2e比率。使用对需氧培养物测定的YmaxATP值计算P/2e比率。当琥珀酸盐作为碳源和能源时，以硝酸盐作为电子受体的硫酸盐限制和琥珀酸盐限制培养物的平均P/2e值分别为0.5和0.7，亚硝酸盐限制培养物的平均P/2e值为0.9。以葡萄糖酸盐作为碳源和能源时，以硝酸盐作为电子受体的葡萄糖酸盐限制培养物和硝酸盐限制培养物的平均P/2e值分别为0.9和1.1。从硫酸盐、琥珀酸盐、亚硝酸盐、葡萄糖酸盐和硝酸盐限制培养物中获得的细胞中测得的H+/O比率，对于内源性底物分别产生3.4、4.5、3.5、4.8和6.2的平均值。根据我们的数据，我们得出结论，硫酸盐和亚硝酸盐限制导致位点I磷酸化的丧失。亚硝酸盐对ATP的最大生长产量没有影响。我们提出，反硝化副球菌异养生长细胞中的代谢是在电子传递链位点I区域的磷酸化水平上进行调节的。
