Naini A B, Vontzalidou E, Côté L J
Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Clin Chem. 1993 Feb;39(2):247-50.
A method for measuring gamma-aminobutyric acid (GABA) in human cerebrospinal fluid (CSF) by isocratic HPLC with electrochemical detection is described. The method is based on precolumn derivatization of GABA with o-phthaldialdehyde (OPA) and tert-butylthiol (t-BT), separation of the GABA-OPA complex on a reversed-phase column, and quantitation by means of a Coulochem electrochemical detector. The method is highly sensitive and specific for GABA. In three samples of human CSF containing low, medium, and high amounts of GABA, the coefficients of variation between and within runs were 4.5% and 3.6%, respectively. The concentration of GABA in 10 neurologically intact subjects was 92.5 +/- 9.4 nmol/L of CSF.
描述了一种采用等度高效液相色谱法（HPLC）并结合电化学检测来测定人脑脊液（CSF）中γ-氨基丁酸（GABA）的方法。该方法基于用邻苯二甲醛（OPA）和叔丁硫醇（t-BT）对GABA进行柱前衍生化，在反相柱上分离GABA-OPA复合物，并通过库仑电化学检测器进行定量。该方法对GABA具有高灵敏度和特异性。在三份分别含有低、中、高含量GABA的人CSF样本中，批间和批内变异系数分别为4.5%和3.6%。10名神经功能正常受试者CSF中GABA的浓度为92.5±9.4 nmol/L。
