Esteban E, Snipes K, Hird D, Kasten R, Kinde H
Department of Epidemiology and Preventive Veterinary Medicine, School of Veterinary Medicine, University of California, Davis 95616.
J Clin Microbiol. 1993 Feb;31(2):233-7. doi: 10.1128/jcm.31.2.233-237.1993.
Forty-five isolates of Salmonella serotype reading, 20 isolates of Salmonella serotype senftenberg, and 56 isolates of Salmonella serotype typhimurium from domestic and wild animals were characterized genotypically to differentiate within serotypes for epidemiologic studies. The genotypic method of characterization used was ribotyping, a method for highlighting highly conserved rRNA genes and associated sequences. Isolates were obtained from diverse geographic sources (farms located in Fresno, Sonoma, Stanislaus, and Yolo counties) as well as different hosts (avian, equine, bovine, murine, and environmental) during a period of 8 months. Within a given serotype, ribotying was able to establish subclassifications (ribotypes) that grouped isolates by a common source regardless of host or geographic origin. There were four distinct ribosomal banding patterns observed for Salmonella serotype reading, six were observed for Salmonella serotype senftenberg, and two were observed for Salmonella serotype typhimurium.
对来自家畜和野生动物的45株雷丁沙门氏菌血清型菌株、20株森夫滕贝格沙门氏菌血清型菌株以及56株鼠伤寒沙门氏菌血清型菌株进行了基因分型，以便在血清型内进行区分，用于流行病学研究。所采用的基因分型鉴定方法是核糖体分型，这是一种突出高度保守的rRNA基因及相关序列的方法。在8个月的时间里，从不同地理来源（位于弗雷斯诺、索诺马、斯坦尼斯劳斯和约洛县的农场）以及不同宿主（禽类、马、牛、鼠类和环境）中获取了菌株。在给定的血清型内，核糖体分型能够建立亚分类（核糖型），将菌株按共同来源分组，而不论宿主或地理来源如何。雷丁沙门氏菌血清型观察到四种不同的核糖体条带模式，森夫滕贝格沙门氏菌血清型观察到六种，鼠伤寒沙门氏菌血清型观察到两种。
