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神经外胚层起源肿瘤的免疫细胞化学检测

Immunocytochemical detection of tumours of neuroectodermal origin.

作者信息

Carey P J, Malcolm A J, Pearson A D, Kemshead J T, Reid M M

机构信息

Department of Haematology, University of Newcastle upon Tyne.

出版信息

J Clin Pathol. 1988 May;41(5):586-9. doi: 10.1136/jcp.41.5.586.

DOI:10.1136/jcp.41.5.586
PMID:3290267
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1141518/
Abstract

Immunocytochemical methods were applied to bone marrow aspirate and cerebrospinal fluid specimens to show cellular reactivity with the monoclonal antibody UJ13A, which recognises an antigen expressed by cells of neuroectodermal origin. The antigen remained stable after air drying and appropriate fixation. In five patients with various neuroectodermal tumours the diagnostic advantages of these techniques were clear; they can be performed even when only very small amounts of diagnostic material are available.

摘要

免疫细胞化学方法应用于骨髓穿刺液和脑脊液标本,以显示与单克隆抗体UJ13A的细胞反应性,该抗体识别神经外胚层来源细胞表达的一种抗原。经空气干燥和适当固定后,该抗原保持稳定。在5例患有各种神经外胚层肿瘤的患者中,这些技术的诊断优势明显;即使只有极少量的诊断材料,也能进行这些检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b09/1141518/28d821dd3c73/jclinpath00337-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b09/1141518/28d821dd3c73/jclinpath00337-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b09/1141518/28d821dd3c73/jclinpath00337-0107-a.jpg

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本文引用的文献

1
Biological characterization and clinical applications of a monoclonal antibody recognizing an antigen restricted to neuroectodermal tissues.一种识别仅限于神经外胚层组织抗原的单克隆抗体的生物学特性及临床应用
Int J Cancer. 1983 May 15;31(5):591-8. doi: 10.1002/ijc.2910310510.
2
The characterisation of monoclonal antibodies against haemopoietic cells: comparison of an immunoperoxidase method with fluorescence activated cell sorting.
J Immunol Methods. 1983 Jul 15;61(2):171-82. doi: 10.1016/0022-1759(83)90160-6.
3
Use of panels of monoclonal antibodies in the differential diagnosis of neuroblastoma and lymphoblastic disorders.单克隆抗体组合在神经母细胞瘤和淋巴细胞性疾病鉴别诊断中的应用。
J Clin Pathol. 1990 Jan;43(1):9-12. doi: 10.1136/jcp.43.1.9.
Lancet. 1983 Jan 1;1(8314-5):12-5. doi: 10.1016/s0140-6736(83)91559-3.
4
A rapid procedure, by collagenase treatment and cytocentrifugation, for the cytological evaluation of bone marrow biopsy specimens.一种通过胶原酶处理和细胞离心技术对骨髓活检标本进行细胞学评估的快速方法。
Clin Lab Haematol. 1986;8(2):149-54. doi: 10.1111/j.1365-2257.1986.tb00089.x.