大肠杆菌细胞色素o复合体CyoA-a亚基周质片段的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of the periplasmic fragment of CyoA-a subunit of the Escherichia coli cytochrome o complex.

作者信息

van der Oost J, Musacchio A, Pauptit R A, Ceska T A, Wierenga R K, Saraste M

机构信息

European Molecular Biology Laboratory, Heidelberg, Germany.

出版信息

J Mol Biol. 1993 Feb 5;229(3):794-6. doi: 10.1006/jmbi.1993.1083.

DOI:10.1006/jmbi.1993.1083

PMID:8433374

Abstract

CyoA, an integral membrane protein, is a subunit of the Escherichia coli cytochrome o quinol oxidase complex. The C-terminal periplasmic domain of CyoA has been expressed in E. coli, purified and crystallized. Crystals were grown using ammonium sulphate as a precipitant. They have space group I222 or I2(1)2(1)2(1) and diffract X-rays to 2.3 A resolution.

摘要

细胞色素 o 泛醌氧化酶 A（CyoA）是一种整合膜蛋白，是大肠杆菌细胞色素 o 泛醌氧化酶复合物的一个亚基。CyoA 的 C 末端周质结构域已在大肠杆菌中表达、纯化并结晶。使用硫酸铵作为沉淀剂培养晶体。它们的空间群为 I222 或 I2(1)2(1)2(1)，X 射线衍射分辨率为 2.3 Å。