Couchman J R, Abrahamson D R, McCarthy K J
Department of Cell Biology, University of Alabama, Birmingham.
Kidney Int. 1993 Jan;43(1):79-84. doi: 10.1038/ki.1993.14.
Basement membranes contain distinct collagen, glycoprotein and proteoglycan species, and these exhibit considerable heterogeneity in isoform or type when different tissue types are compared. Additionally, many components are differentially expressed in organogenesis. We have considered the distributions in glomerulogenesis of two distinct basement membrane proteoglycans, a small heparan sulfate proteoglycan and a chondroitin sulfate proteoglycan (BM-CSPG). While the former was present in all kidney basement membranes through development, the latter was apparently regulated in distribution. BM-CSPG was only strongly expressed in the vasculature invading late comma stage glomeruli, and later in presumptive and mature Bowman's capsule. Over the first six to eight weeks, the capillary basement membranes contained BM-CSPG, but in gradually decreasing amounts until it became completely undetectable. The basement membrane of the adult rat glomerulus is unique in its lack of BM-CSPG. However, in diabetic rats, BM-CSPG is apparently re-expressed in the glomerular basement membrane, a potential marker for pathological changes in glomerular structure. While its function awaits elucidation, BM-CSPG may be essential for basement membrane integrity or stability and have important roles in kidney development.
基底膜包含不同的胶原蛋白、糖蛋白和蛋白聚糖种类，当比较不同组织类型时，这些在异构体或类型上表现出相当大的异质性。此外，许多成分在器官发生过程中差异表达。我们研究了两种不同的基底膜蛋白聚糖在肾小球发生过程中的分布，一种是小分子硫酸乙酰肝素蛋白聚糖，另一种是硫酸软骨素蛋白聚糖（BM-CSPG）。在整个发育过程中，前者存在于所有肾基底膜中，而后者的分布显然受到调控。BM-CSPG仅在侵入晚期逗号期肾小球的血管中强烈表达，随后在假定的和成熟的鲍曼囊中表达。在最初的六到八周内，毛细血管基底膜含有BM-CSPG，但含量逐渐减少，直至完全无法检测到。成年大鼠肾小球的基底膜独特之处在于缺乏BM-CSPG。然而，在糖尿病大鼠中，BM-CSPG显然在肾小球基底膜中重新表达，这是肾小球结构病理变化的一个潜在标志物。虽然其功能尚待阐明，但BM-CSPG可能对基底膜的完整性或稳定性至关重要，并在肾脏发育中发挥重要作用。
