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两种光转导膜蛋白：细菌视紫红质和哺乳动物视紫红质。

Two light-transducing membrane proteins: bacteriorhodopsin and the mammalian rhodopsin.

作者信息

Khorana H G

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1166-71. doi: 10.1073/pnas.90.4.1166.

DOI:10.1073/pnas.90.4.1166
PMID:8433978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC45834/
Abstract

Site-directed mutagenesis has provided insight into the mechanisms of action of bacteriorhodopsin and rhodopsin. These studies are summarized here.

摘要

定点诱变技术为深入了解细菌视紫红质和视紫红质的作用机制提供了线索。以下是对这些研究的总结。

相似文献

1
Two light-transducing membrane proteins: bacteriorhodopsin and the mammalian rhodopsin.两种光转导膜蛋白：细菌视紫红质和哺乳动物视紫红质。
Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1166-71. doi: 10.1073/pnas.90.4.1166.
2
Signal transduction in two light-transducing systems: bacteriorhodopsin and mammalian rhodopsin.两种光转导系统中的信号转导：细菌视紫红质和哺乳动物视紫红质。
Nucleic Acids Symp Ser. 1993(29):219.
3
Hydrophobic amino acids in the retinal-binding pocket of bacteriorhodopsin.细菌视紫红质视网膜结合口袋中的疏水氨基酸。
J Biol Chem. 1993 Sep 25;268(27):20305-11.
4
Bacteriorhodopsin chimeras containing the third cytoplasmic loop of bovine rhodopsin activate transducin for GTP/GDP exchange.含有牛视紫红质第三胞质环的细菌视紫红质嵌合体激活转导蛋白以进行GTP/GDP交换。
Protein Sci. 2006 Jul;15(7):1679-90. doi: 10.1110/ps.062192306.
5
Modulation of GDP release from transducin by the conserved Glu134-Arg135 sequence in rhodopsin.视紫红质中保守的Glu134-Arg135序列对转导蛋白释放GDP的调节作用。
J Biol Chem. 1996 Oct 11;271(41):25406-11. doi: 10.1074/jbc.271.41.25406.
6
Detection and expression of a gene encoding a new bacteriorhodopsin from an extreme halophile strain HT (JCM 9743) which does not possess bacteriorhodopsin activity.从一株不具有细菌视紫红质活性的嗜极嗜盐菌HT（JCM 9743）中检测并表达一种编码新型细菌视紫红质的基因。
Extremophiles. 1998 Jan;2(1):33-9. doi: 10.1007/s007920050040.
7
Heterogeneity of rhodopsin intermediate state interacting with transducin.视紫红质中间态与转导蛋白相互作用的异质性。
Methods Enzymol. 2000;315:347-63. doi: 10.1016/s0076-6879(00)15853-7.
8
Structure and function in rhodopsin. Single cysteine substitution mutants in the cytoplasmic interhelical E-F loop region show position-specific effects in transducin activation.视紫红质的结构与功能。胞质内螺旋间E-F环区域的单半胱氨酸取代突变体在转导蛋白激活中表现出位置特异性效应。
Biochemistry. 1996 Sep 24;35(38):12464-9. doi: 10.1021/bi960848t.
9
Photochemical reaction cycle and proton transfers in Neurospora rhodopsin.粗糙脉孢菌视紫红质中的光化学反应循环与质子转移
J Biol Chem. 2001 Aug 31;276(35):32495-505. doi: 10.1074/jbc.M102652200. Epub 2001 Jul 2.
10
Hydrophobic amino acids at the cytoplasmic ends of helices 3 and 6 of rhodopsin conjointly modulate transducin activation.螺旋 3 和 6 的细胞质末端的疏水性氨基酸共同调节转导蛋白的激活。
Arch Biochem Biophys. 2011 Feb 15;506(2):142-9. doi: 10.1016/j.abb.2010.11.019. Epub 2010 Nov 27.

引用本文的文献

1
Expression systems for bovine rhodopsin: a review of the progress made in the Khorana laboratory.牛视紫红质表达系统：科拉纳实验室取得的进展综述
Biophys Rev. 2023 Jan 6;15(1):93-101. doi: 10.1007/s12551-022-01037-2. eCollection 2023 Feb.
2
Protein Design: From the Aspect of Water Solubility and Stability.蛋白质设计：从水溶性和稳定性方面考虑。
Chem Rev. 2022 Sep 28;122(18):14085-14179. doi: 10.1021/acs.chemrev.1c00757. Epub 2022 Aug 3.
3
Biophysical Reviews contribution call: an issue focus on the life and works of Prof. Har Gobind Khorana on the occasion of the 100th anniversary of the year of his birth.

本文引用的文献

1
Light-induced interaction between rhodopsin and the GTP-binding protein. Metarhodopsin II is the major photoproduct involved.视紫红质与GTP结合蛋白之间的光诱导相互作用。主要的光产物是变视紫红质II。
Eur J Biochem. 1982 Sep;127(1):97-103. doi: 10.1111/j.1432-1033.1982.tb06842.x.
2
Bacteriorhodopsin and related pigments of halobacteria.嗜盐菌的细菌视紫红质及相关色素。
Annu Rev Biochem. 1982;51:587-616. doi: 10.1146/annurev.bi.51.070182.003103.
3
The molecular basis of visual excitation.视觉兴奋的分子基础。
《生物物理评论》征稿启事：为纪念哈·戈宾德·科拉纳教授诞辰100周年，特推出一期聚焦其生平与著作的专刊。
Biophys Rev. 2022 May 23;14(3):611-612. doi: 10.1007/s12551-022-00958-2. eCollection 2022 Jun.
4
Photonic Potential of Haloarchaeal Pigment Bacteriorhodopsin for Future Electronics: A Review.嗜盐古菌色素细菌视紫红质在未来电子学中的光子潜力：综述
Curr Microbiol. 2017 Aug;74(8):996-1002. doi: 10.1007/s00284-017-1271-5. Epub 2017 Jun 2.
5
Light-driven Na(+) pump from Gillisia limnaea: a high-affinity Na(+) binding site is formed transiently in the photocycle.来自湖沼吉氏菌的光驱动钠泵：在光循环中会短暂形成一个高亲和力的钠结合位点。
Biochemistry. 2014 Dec 9;53(48):7549-61. doi: 10.1021/bi501064n. Epub 2014 Nov 24.
6
Tripod Amphiphiles for Membrane Protein Manipulation.用于膜蛋白操作的三脚架两亲分子
Mol Biosyst. 2010;6:89-94. doi: 10.1039/b915162c.
7
Breaking the carboxyl rule: lysine 96 facilitates reprotonation of the Schiff base in the photocycle of a retinal protein from Exiguobacterium sibiricum.打破羧基规则：赖氨酸 96 有利于西伯利亚极端嗜热杆菌视蛋白光循环中席夫碱的质子化。
J Biol Chem. 2013 Jul 19;288(29):21254-21265. doi: 10.1074/jbc.M113.465138. Epub 2013 May 21.
8
Channelrhodopsin-1 initiates phototaxis and photophobic responses in chlamydomonas by immediate light-induced depolarization.通道视紫红质-1通过光诱导的即时去极化引发衣藻的趋光性和避光反应。
Plant Cell. 2008 Jun;20(6):1665-77. doi: 10.1105/tpc.108.057919. Epub 2008 Jun 13.
9
An improved tripod amphiphile for membrane protein solubilization.一种用于膜蛋白增溶的改良三脚架两亲分子。
Protein Sci. 2000 Dec;9(12):2518-27. doi: 10.1110/ps.9.12.2518.
10
The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins.粗糙脉孢菌的nop-1基因编码一种与古细菌视紫红质同源的七跨膜螺旋视网膜结合蛋白。
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8034-9. doi: 10.1073/pnas.96.14.8034.
Nature. 1968 Aug 24;219(5156):800-7. doi: 10.1038/219800a0.
4
Structure-function studies on bacteriorhodopsin. V. Effects of amino acid substitutions in the putative helix F.细菌视紫红质的结构-功能研究。V. 假定的F螺旋中氨基酸取代的影响。
J Biol Chem. 1987 Jul 5;262(19):9277-84.
5
Total synthesis of a gene for bovine rhodopsin.牛视紫红质基因的全合成
Proc Natl Acad Sci U S A. 1986 Feb;83(3):599-603. doi: 10.1073/pnas.83.3.599.
6
Aspartic acid substitutions affect proton translocation by bacteriorhodopsin.天冬氨酸替代影响细菌视紫红质的质子转运。
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4148-52. doi: 10.1073/pnas.85.12.4148.
7
Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin.半胱氨酸残基110和187对于牛视紫红质正确结构的形成至关重要。
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8459-63. doi: 10.1073/pnas.85.22.8459.
8
Bacteriorhodopsin mutants containing single tyrosine to phenylalanine substitutions are all active in proton translocation.含有单个酪氨酸到苯丙氨酸取代的细菌视紫红质突变体在质子转运中均具有活性。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5595-9. doi: 10.1073/pnas.84.16.5595.
9
Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments.当视紫红质被磷酸化并结合视杆外段的内在48 kDa蛋白时，光激发视紫红质引起的磷酸二酯酶激活被淬灭。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1174-8. doi: 10.1073/pnas.83.5.1174.
10
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.合成牛视紫红质基因在猴肾细胞中的表达。
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8874-8. doi: 10.1073/pnas.84.24.8874.