使用新型靶标暴露液检测人癌常规石蜡包埋组织中的p53过表达。
Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid.
作者信息
van den Berg F M, Baas I O, Polak M M, Offerhaus G J
机构信息
Department of Pathology, University of Amsterdam, The Netherlands.
出版信息
Am J Pathol. 1993 Feb;142(2):381-5.
With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualized by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb1801 as well as polyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.
借助新开发的靶标揭显液（TUF），通过免疫组织化学在结肠、胃和胰腺肿瘤的近期和存档石蜡包埋组织样本上可视化p53过表达。使用单克隆抗p53抗体pAb1801以及针对p53 CM1的多克隆抗血清，与来自相同肿瘤的新鲜冷冻组织相比，TUF介导的免疫组织化学在石蜡包埋癌样本中对p53过表达完全一致。因此，通过TUF介导的单克隆和多克隆抗体免疫组织化学对常规用福尔马林固定并石蜡包埋的恶性组织中p53过表达进行预后和诊断评估，可作为诊断和研究组织病理学的一种新工具。
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