Reitamo S, Remitz A, Varga J, Ceska M, Effenberger F, Jimenez S, Uitto J
Department of Dermatology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa. 19107.
Arch Dermatol. 1993 Feb;129(2):189-93.
Interleukin 8 (IL-8), a chemotactic cytokine produced by various cell types, displays structural homology to the connective tissue-activating peptide III. Little is known of the possible role of IL-8 in connective tissue disorders. We therefore determined serum concentrations of IL-8 and autoantibodies to IL-8 in 134 patients with systemic sclerosis (SSc) and related connective tissue disorders, as well as in pooled serum from 28 healthy control subjects by a sensitive enzyme-linked immunosorbent assay.
Interleukin 8 was undetectable in the pooled serum from 28 healthy controls, but detectable in serum samples from 24 of the 134 patients described above. It was detected in 13 of 60 patients with limited SSc and in eight of 48 patients with diffuse SSc. It was also detectable in one of three patients with eosinophilic fasciitis and in two of 10 patients with Raynaud's syndrome without skin involvement. In contrast, none of the three patients with morphea or the 10 patients with eosinophilia-myalgia syndrome had detectable IL-8 levels. We further determined the concentration of autoantibodies to IL-8 in the same serum samples. The values in healthy controls were 6.7 +/- 0.2 ng/mL (mean +/- SEM). Significantly elevated autoantibody levels were detected in patients with limited SSc (21.5 +/- 1.7), diffuse SSc (23.4 +/- 2.2), and Raynaud's syndrome (20.5 +/- 3.7). Elevated levels were also detected in patients with eosinophilic fasciitis (43.7 +/- 8.6) and morphea (14.7 +/- 3.2). Normal levels (7.5 +/- 2.0) were found in patients with eosinophilia-myalgia syndrome. Analysis of variance between the levels of autoantibodies to IL-8 and duration of the disease, extent of skin involvement, drug therapy, or serologic findings failed to show a significant correlation.
These results suggest that increased production of IL-8 may relate to activation of mononuclear phagocytes, fibroblasts, or endothelial cells, among other cell types, in patients with SSc, but not in those with eosinophilia-myalgia syndrome. This activation could be related to the production of autoantibodies to IL-8.
白细胞介素8（IL-8）是一种由多种细胞类型产生的趋化性细胞因子，与结缔组织激活肽III具有结构同源性。关于IL-8在结缔组织疾病中可能发挥的作用，人们了解甚少。因此，我们采用灵敏的酶联免疫吸附测定法，测定了134例系统性硬化症（SSc）及相关结缔组织疾病患者以及28例健康对照者混合血清中的IL-8血清浓度和抗IL-8自身抗体。
在28例健康对照者的混合血清中未检测到白细胞介素8，但在上述134例患者的血清样本中，有24例可检测到。在60例局限性SSc患者中有13例检测到，在4例弥漫性SSc患者中有8例检测到。在3例嗜酸性筋膜炎患者中有1例可检测到，在10例无皮肤受累的雷诺综合征患者中有2例可检测到。相比之下，3例硬斑病患者和10例嗜酸性粒细胞增多性肌痛综合征患者中均未检测到可检测到的IL-8水平。我们进一步测定了同一血清样本中抗IL-8自身抗体的浓度。健康对照者的值为6.7±0.2 ng/mL（平均值±标准误）。在局限性SSc患者（21.5±1.7）、弥漫性SSc患者（23.4±2.2）和雷诺综合征患者（20.5±3.7）中检测到自身抗体水平显著升高。嗜酸性筋膜炎患者（43.7±8.6）和硬斑病患者（14.7±3.2）中也检测到水平升高。嗜酸性粒细胞增多性肌痛综合征患者的水平正常（7.5±2.0）。对IL-8自身抗体水平与疾病持续时间？皮肤受累程度？药物治疗或血清学检查结果之间进行方差分析，未显示出显著相关性。
这些结果表明，IL-8产生增加可能与SSc患者而非嗜酸性粒细胞增多性肌痛综合征患者的单核吞噬细胞？成纤维细胞或内皮细胞等其他细胞类型的激活有关。这种激活可能与抗IL-8自身抗体的产生有关。
