利用杆状病毒系统表达人血栓素合酶。
Expression of human thromboxane synthase using a baculovirus system.
作者信息
Yokoyama C, Miyata A, Suzuki K, Nishikawa Y, Yoshimoto T, Yamamoto S, Nüsing R, Ullrich V, Tanabe T
机构信息
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
出版信息
FEBS Lett. 1993 Feb 22;318(1):91-4. doi: 10.1016/0014-5793(93)81335-w.
Human thromboxane (TX) synthase (EC 5.3.99.5) was produced by the baculovirus expression system using cDNA encoding human TX synthase [(1991) Biochem. Biophys. Res. Commun. 78, 1479-1484]. A recombinant baculovirus TXS7 was expressed in Spodoptera frugiperda Sf9 insect cells. The expressed protein was recognized by monoclonal antibody, Kon 7 raised against human TX synthase [(1990) Blood 76, 80-85]. The recombinant TX synthase catalyzed the conversion of prostaglandin (PG) H2 to TXA2 and 12-hydroxy-heptadecatrienoic acid (HHT). Both conversions of PGH2 to TXA2 and HHT by the expressed TX synthase were completely inhibited by a specific TX synthase inhibitor, OKY-046 (5 microM).
人血栓素（TX）合酶（EC 5.3.99.5）是通过杆状病毒表达系统利用编码人TX合酶的cDNA制备的[（1991年）《生物化学与生物物理研究通讯》78卷，第1479 - 1484页]。重组杆状病毒TXS7在草地贪夜蛾Sf9昆虫细胞中表达。表达的蛋白可被针对人TX合酶制备的单克隆抗体Kon 7识别[（1990年）《血液》76卷，第80 - 85页]。重组TX合酶催化前列腺素（PG）H2转化为血栓素A2（TXA2）和12 - 羟基 - 十七碳三烯酸（HHT）。表达的TX合酶将PGH2转化为TXA2和HHT的过程均被特异性TX合酶抑制剂OKY - 046（5微摩尔）完全抑制。
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