秀丽隐杆线虫中RNA聚合酶II水平的转录后调控
Post-transcriptional regulation of RNA polymerase II levels in Caenorhabditis elegans.
作者信息
Dalley B K, Rogalski T M, Tullis G E, Riddle D L, Golomb M
机构信息
Division of Biological Sciences University of Missouri-Columbia 65211.
出版信息
Genetics. 1993 Feb;133(2):237-45. doi: 10.1093/genetics/133.2.237.
Abstract
To investigate the regulation of RNA polymerase II levels in Caenorhabditis elegans, we have constructed nematode strains having one, two, or three copies of ama-1, the gene for the largest subunit of RNA polymerase II. Steady-state levels of RNA polymerase II polypeptides and solubilized enzyme activity are invariant with gene dosage, indicating regulatory compensation. However, steady-state levels of ama-1 mRNA are directly proportional to gene dosage. These results imply that RNA polymerase II levels in C. elegans are regulated post-transcriptionally.
摘要
为了研究秀丽隐杆线虫中RNA聚合酶II水平的调控，我们构建了具有一份、两份或三份ama-1（RNA聚合酶II最大亚基的基因）拷贝的线虫菌株。RNA聚合酶II多肽的稳态水平和可溶酶活性不随基因剂量而变化，表明存在调控补偿。然而，ama-1 mRNA的稳态水平与基因剂量成正比。这些结果表明秀丽隐杆线虫中的RNA聚合酶II水平是在转录后受到调控的。
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