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与酿酒酵母RNA14的同源性表明，果蝇中叉状抑制因子对表型的抑制发生在RNA稳定性水平。

Homology with Saccharomyces cerevisiae RNA14 suggests that phenotypic suppression in Drosophila melanogaster by suppressor of forked occurs at the level of RNA stability.

作者信息

Mitchelson A, Simonelig M, Williams C, O'Hare K

机构信息

Department of Biochemistry, Imperial College of Science Technology and Medicine, London, UK.

出版信息

Genes Dev. 1993 Feb;7(2):241-9. doi: 10.1101/gad.7.2.241.

DOI:10.1101/gad.7.2.241
PMID:8436295
Abstract

The suppressor of forked [su(f)] locus of Drosophila melanogaster encodes at least one cell-autonomous vital function. Mutations at su(f) can affect the expression of unlinked genes where retroviral-like transposable elements are inserted. Changes in phenotype are correlated with changes in mRNA profiles, indicating that su(f) affects the production and/or stability of mRNAs. We have cloned the su(f) gene by P-element transposon tagging. Alterations in the DNA map of eight lethal alleles were detected in a 4.3-kb region. P-element-mediated transformation using a fragment including this interval rescued all aspects of the su(f) mutant phenotype. The gene is transcribed to produce a major 2.6-kb RNA and minor RNAs of 1.3 and 2.9 kb, which are present throughout development, being most abundant in embryos, pupae, and adult females. The major predicted gene product is an 84- kD protein that is homologous to RNA14 of Saccharomyces cerevisiae, a vital gene where mutation affects mRNA stability. This suggests that phenotypic modification by su(f) occurs at the level of RNA stability.

摘要

黑腹果蝇的叉状抑制基因[su(f)]编码至少一种细胞自主的重要功能。su(f)位点的突变会影响插入了逆转录病毒样转座元件的非连锁基因的表达。表型变化与mRNA谱的变化相关，这表明su(f)影响mRNA的产生和/或稳定性。我们通过P因子转座子标签法克隆了su(f)基因。在一个4.3 kb的区域内检测到了八个致死等位基因的DNA图谱变化。使用包含该区间的片段进行P因子介导的转化挽救了su(f)突变体表型的所有方面。该基因转录产生一个主要的2.6 kb RNA以及1.3 kb和2.9 kb的次要RNA，它们在整个发育过程中都存在，在胚胎、蛹和成年雌性中最为丰富。预测的主要基因产物是一种84 kD的蛋白质，它与酿酒酵母的RNA14同源，酿酒酵母中的一个重要基因，其突变会影响mRNA的稳定性。这表明su(f)引起的表型修饰发生在RNA稳定性水平。

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