果蝇叉状位点抑制子处的等位基因间互补揭示了在不同区域发生突变的叉状抑制子蛋白之间的互补作用。
Interallelic complementation at the suppressor of forked locus of Drosophila reveals complementation between suppressor of forked proteins mutated in different regions.
作者信息
Simonelig M, Elliott K, Mitchelson A, O'Hare K
机构信息
Institut Jacques Monod, Université Paris, France.
出版信息
Genetics. 1996 Apr;142(4):1225-35. doi: 10.1093/genetics/142.4.1225.
Abstract
The Su(f) protein of Drosophila melanogaster shares extensive homologies with proteins from yeast (RNA14) and man (77 kD subunit of cleavage stimulation factor) that are required for 3' end processing of mRNA. These homologies suggest that su(f) is involved in mRNA 3' end formation and that some aspects of this process are conserved throughout eukaryotes. We have investigated the genetic and molecular complexity of the su(f) locus. The su(f) gene is transcribed to produce three RNAs and could encode two proteins. Using constructs that contain different parts of the locus, we show that only the larger predicted gene product of 84 kD is required for the wild-type function of su(f). Some lethal alleles of su(f) complement to produce viable combinations. The structures of complementing and noncomplementing su(f) alleles indicate that 84-kD Su(f) proteins mutated in different domains can act in combination for partial su(f) function. Our results suggest protein-protein interaction between or within wild-type Su(f) molecules.
摘要
黑腹果蝇的Su(f)蛋白与酵母（RNA14）和人类（切割刺激因子的77 kD亚基）中参与mRNA 3'端加工所需的蛋白质具有广泛的同源性。这些同源性表明，su(f)参与mRNA 3'端的形成，并且这一过程的某些方面在整个真核生物中是保守的。我们研究了su(f)基因座的遗传和分子复杂性。su(f)基因转录产生三种RNA，可能编码两种蛋白质。使用包含该基因座不同部分的构建体，我们表明，对于su(f)的野生型功能，仅需要预测的较大的84 kD基因产物。su(f)的一些致死等位基因互补产生可行的组合。互补和非互补的su(f)等位基因的结构表明，在不同结构域发生突变的84-kD Su(f)蛋白可以组合发挥部分su(f)功能。我们的结果表明野生型Su(f)分子之间或内部存在蛋白质-蛋白质相互作用。
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