Martini N, Egen M, Rüntz I, Strittmatter G
Abteilung Biochemie, Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Mol Gen Genet. 1993 Jan;236(2-3):179-86. doi: 10.1007/BF00277110.
Transcription of at least one member of the potato prp1 gene family, prp1-1, is activated at early stages of potato infection with the late blight fungus Phytophthora infestans. In this paper we present evidence that mRNA encoded by prp1-1 does not accumulate in response to abiotic environmental cues which stimulate transcription of other defence-related genes. Regulatory elements were identified in the 5' terminal region of prp1-1 by assaying the expression pattern of chimeric promoter/beta-glucuronidase gene constructs in transgenic potato. A 273 bp fragment comprising the promoter sequence between positions -402 and -130 was sufficient for rapid and strictly localized transcriptional activation at infection sites during the development of late blight disease. Like the native promoter, this truncated promoter did not mediate transcriptional activation in response to other abiotic stimuli. The use of the identified regulatory region to generate conditional mutations selectively at infection sites is discussed.
马铃薯prp1基因家族的至少一个成员prp1-1的转录，在马铃薯被晚疫病菌致病疫霉感染的早期阶段被激活。在本文中，我们提供证据表明，prp1-1编码的mRNA不会因刺激其他防御相关基因转录的非生物环境信号而积累。通过检测嵌合启动子/β-葡萄糖醛酸酶基因构建体在转基因马铃薯中的表达模式，在prp1-1的5'末端区域鉴定出调控元件。一个包含-402至-130位之间启动子序列的273 bp片段，足以在晚疫病发展过程中在感染部位实现快速且严格定位的转录激活。与天然启动子一样，这个截短的启动子不会介导对其他非生物刺激的转录激活。本文还讨论了利用鉴定出的调控区域在感染部位选择性产生条件突变的方法。
