Boerrigter M E, van Duijn C M, Mullaart E, Eikelenboom P, van der Togt C M, Knook D L, Hofman A, Vijg J
Department of Molecular Biology, TNO Institute for Experimental Gerontology, Rijswijk, The Netherlands.
Neurobiol Aging. 1991 Jul-Aug;12(4):367-70. doi: 10.1016/0197-4580(91)90024-e.
Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 +/- 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 +/- 8.2% and 43.0 +/- 4.4%, respectively; p greater than 0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 +/- 5.8%, which was significantly lower than that found in controls (p less than 0.01) or in the other AD patients (p less than 0.02).
我们采用碱性滤膜洗脱技术，对43例阿尔茨海默病（AD）患者及48名年龄、性别匹配的正常健康对照者新鲜分离的外周血淋巴细胞（PBL）进行体外N-亚硝基-N-乙基脲（ENU）暴露后，检测DNA单链断裂（SSB）的诱导及消失情况。ENU处理1小时后，对照者PBL中SSB消失的平均百分比为41.4±2.9%；这与无（n = 16）或有一名（n = 12）痴呆一级亲属的AD患者样本中的结果无显著差异（分别为42.5±8.2%和43.0±4.4%；p＞0.75）。然而，在至少有两名痴呆一级亲属的15例AD患者的PBL中，SSB消失的平均百分比为23.6±5.8%，显著低于对照者（p＜0.01）或其他AD患者（p＜0.02）。
