Fisher R A, Newlands E S
Department of Medical Oncology, Charing Cross and Westminster Medical School, London, England.
Am J Obstet Gynecol. 1993 Feb;168(2):563-9. doi: 10.1016/0002-9378(93)90494-4.
The purpose of our study was to determine whether the polymerase chain reaction can be used to diagnose the gestational trophoblastic disease hydatidiform mole and distinguish between partial, monospermic complete, and dispermic complete hydatidiform mole.
In 20 cases of well-characterized hydatidiform mole, deoxyribonucleic acid was amplified from parental and molar samples by using primers for the variable number tandem repeat sequence YNZ22 and for sex chromosome-specific sequences.
Polymerase chain reaction amplification of the YNZ22 polymorphism identified contributions from both parents in 5 of 7 partial hydatidiform moles. Nine of 13 complete hydatidiform moles were shown to be androgenetic by using primers for the YNZ22 polymorphism. Two of the complete hydatidiform moles were classified as dispermic on the basis of Y chromosome-specific sequences.
Polymerase chain reaction was shown to be a rapid and accurate method of identifying parental contributions to the molar genome and thus has the potential to be used for diagnosis and classification of hydatidiform moles.
我们研究的目的是确定聚合酶链反应是否可用于诊断妊娠滋养细胞疾病葡萄胎，并区分部分性、单精子完全性和双精子完全性葡萄胎。
在20例特征明确的葡萄胎病例中，通过使用针对可变数目串联重复序列YNZ22和性染色体特异性序列的引物，从亲代和葡萄胎样本中扩增脱氧核糖核酸。
YNZ22多态性的聚合酶链反应扩增显示，7例部分性葡萄胎中有5例的基因组来自双亲。利用YNZ22多态性引物，13例完全性葡萄胎中有9例显示为孤雄来源。根据Y染色体特异性序列，其中2例完全性葡萄胎被分类为双精子性。
聚合酶链反应被证明是一种快速、准确的方法，可用于识别亲代对葡萄胎基因组的贡献，因此有潜力用于葡萄胎的诊断和分类。
