Datta A R, Moore M A, Wentz B A, Lane J
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Appl Environ Microbiol. 1993 Jan;59(1):144-9. doi: 10.1128/aem.59.1.144-149.1993.
A plasmid containing the cloned listeriolysin gene of Listeria monocytogenes was used as a probe to identify Listeria strains by DNA colony hybridization. The probe DNA was labeled with horseradish peroxidase in the presence of glutaraldehyde. After the hybridization and wash procedures, the hybrid molecules were detected by luminescence, which resulted from the oxidation of luminol by a horseradish peroxidase-hydrogen peroxide-coupled reaction. Of the 150 Listeria strains and 16 non-Listeria strains examined, the probe hybridized only with L. monocytogenes. The technique was also used to enumerate L. monocytogenes in artificially contaminated foods.
一个含有单核细胞增生李斯特菌克隆溶血素基因的质粒被用作探针，通过DNA菌落杂交来鉴定李斯特菌菌株。在戊二醛存在的情况下，探针DNA用辣根过氧化物酶进行标记。杂交和洗涤程序完成后，通过发光检测杂交分子，发光是由辣根过氧化物酶-过氧化氢偶联反应氧化鲁米诺产生的。在所检测的150株李斯特菌菌株和16株非李斯特菌菌株中，该探针仅与单核细胞增生李斯特菌杂交。该技术还用于对人工污染食品中的单核细胞增生李斯特菌进行计数。
