Bohnert M, Dilasser F, Dalet C, Mengaud J, Cossart P
LCHA-CNEVA, Paris.
Res Microbiol. 1992 Mar-Apr;143(3):271-80. doi: 10.1016/0923-2508(92)90019-k.
Two 18-mer oligonucleotides derived from the sequence of hly, the gene coding for listeriolysin O, were shown to be specific for Listeria monocytogenes in the genus Listeria in colony hybridization tests. The oligonucleotides did not hybridize with any of the bacterial species found in food and co-isolated with Listeria on selective media. They were used in colony hybridization tests for enumeration of L. monocytogenes present in food samples after direct plating on selective media plates. In addition, two 24-mer oligonucleotides, each including the sequence of one of the 18-mers, were successfully used for the PCR-based detection of L. monocytogenes bacilli present in food samples after 48-h enrichment period. Using this technique, as little as 10(2) bacteria per ml of enrichment broth can be detected.
在菌落杂交试验中，从编码溶血素O的hly基因序列衍生而来的两条18聚体寡核苷酸，被证明对李斯特菌属中的单核细胞增生李斯特菌具有特异性。这些寡核苷酸与在食品中发现的、在选择性培养基上与李斯特菌共分离的任何细菌种类均不杂交。它们被用于菌落杂交试验，以对直接接种在选择性培养基平板上的食品样品中存在的单核细胞增生李斯特菌进行计数。此外，两条24聚体寡核苷酸（每条均包含一条18聚体的序列）在48小时富集期后成功用于基于PCR的食品样品中单核细胞增生李斯特菌杆菌的检测。使用该技术，每毫升富集肉汤中低至10²个细菌都能被检测到。
