Monna L, Omori T, Kodama T
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Appl Environ Microbiol. 1993 Jan;59(1):285-9. doi: 10.1128/aem.59.1.285-289.1993.
Staphylococcus auriculans DBF63, which can grow on dibenzofuran (DBF) or fluorene (FN) as the sole source of carbon and energy, was isolated. Salicylic acid and gentisic acid accumulated in the culture broth of this strain when DBF was supplied as a growth substrate. Also, the formation of 9-fluorenol, 9-fluorenone, 4-hydroxy-9-fluorenone, and 1-hydroxy-9-fluorenone was demonstrated, and accumulation of 1,1a-dihydroxy-1-hydro-9-fluorenone was observed when this strain grew on FN. On the basis of these results, the degradation pathways of DBF and FN were proposed. The analogous oxidation products of dibenzo-p-dioxin were obtained by incubation with DBF-grown S. auriculans DBF63 cells.
分离出了耳葡萄球菌DBF63，它能够以二苯并呋喃（DBF）或芴（FN）作为唯一碳源和能源生长。当以DBF作为生长底物时，水杨酸和龙胆酸在该菌株的培养液中积累。此外，还证实了9-芴醇、9-芴酮、4-羟基-9-芴酮和1-羟基-9-芴酮的形成，并且当该菌株在FN上生长时观察到1,1a-二羟基-1-氢-9-芴酮的积累。基于这些结果，提出了DBF和FN的降解途径。通过与在DBF上生长的耳葡萄球菌DBF63细胞一起培养，获得了二苯并对二恶英的类似氧化产物。
