McCabe R P, Secrist H, Botney M, Egan M, Peters M G
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Clin Immunol Immunopathol. 1993 Jan;66(1):52-8. doi: 10.1006/clin.1993.1007.
Cytokines are involved in the regulation of normal immune events and may be important in the development or perpetuation of immune events in inflammatory bowel disease. We have previously shown that normal human mononuclear cells from tonsil, spleen, and peripheral blood exhibit tissue and stimulus-specific patterns of cytokine mRNA expression. The aim of this study was to determine if disease-dependent differences of cytokine mRNA expression could be found in the intestine. Total RNA was isolated from intestinal mucosa and lamina propria mononuclear cells from inflammatory bowel disease patients and controls. cDNA probes specific for interleukins (IL)-1, -4, -5, and -6 and transforming growth factor-beta were used. IL-1 beta mRNA and TGF-beta mRNA steady state expressions were higher in inflammatory bowel disease specimens than in normal intestine. In addition, mononuclear cell specimens had stronger cytokine mRNA expression than mucosal specimens. The steady state mRNA expression of proinflammatory cytokines is higher in inflammatory bowel disease, consistent with the ongoing inflammation seen.
细胞因子参与正常免疫反应的调节，并且在炎症性肠病免疫反应的发生或持续过程中可能起重要作用。我们之前已经表明，来自扁桃体、脾脏和外周血的正常人单核细胞呈现出细胞因子mRNA表达的组织和刺激特异性模式。本研究的目的是确定在肠道中是否能发现细胞因子mRNA表达的疾病依赖性差异。从炎症性肠病患者和对照组的肠黏膜及固有层单核细胞中分离总RNA。使用了针对白细胞介素（IL）-1、-4、-5和-6以及转化生长因子-β的cDNA探针。炎症性肠病标本中IL-1β mRNA和TGF-β mRNA的稳态表达高于正常肠道。此外，单核细胞标本的细胞因子mRNA表达比黏膜标本更强。促炎细胞因子的稳态mRNA表达在炎症性肠病中更高，这与所观察到的持续炎症一致。
