Merelli E, Sola P, Marasca R, Salati R, Torelli G
Neurological Department, University of Modena, Italy.
Eur Neurol. 1993;33(1):23-6. doi: 10.1159/000116894.
To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences.
为了探究人类嗜T细胞淋巴细胞病毒（HTLV）家族中的逆转录病毒是否可能参与多发性硬化症（MS）的发病过程这一尚未确定的问题，我们通过聚合酶链反应（PCR）检测了30例MS患者和15例慢性进行性脊髓病患者外周血单个核细胞DNA中HTLV-I和HTLV-II序列的存在情况。此外，还检测了14名献血者组成的对照组。所有这些患者的血清和脑脊液在ELISA检测中均未检测到抗HTLV-I抗体。对于PCR，使用了针对HTLV-I和HTLV-II共有的tax区域、HTLV-I的pol区域以及HTLV-II的pol区域的特异性引物和探针。尽管所使用的技术具有很高的灵敏度，但三组受试者的HTLV-I和HTLV-II基因组序列均为阴性。
