Laboratório de Biologia Celular e Molecular, NUPEB, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, Ouro Preto, MG, 35.400-000, Brazil.
Instituto de Ciência e Inovação em Bio-Sustentabilidade (IB-S)/Centro de Biologia Molecular e Ambiental (CBMA), Universidade do Minho, Braga, Portugal.
J Ind Microbiol Biotechnol. 2019 May;46(5):709-723. doi: 10.1007/s10295-018-02119-5. Epub 2019 Jan 24.
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
异常威克汉姆酵母（Wickerhamomyces anomalus）菌株 LBCM1105 最初从甘蔗烧酒（巴西发酵甘蔗汁制成的巴西烈酒）的麦汁中分离得到，该酵母在大量甘油存在下的生长情况异常良好。这种生物柴油工业的主要副产物是酵母生物技术中很有前途的廉价碳源。本文介绍了评估异常威克汉姆酵母利用甘油能力的生理特性，鉴定了一种新的 WaStl1 浓缩甘油-H 协同转运蛋白，其对甘油的亲和力是酿酒酵母的两倍。与该酵母一样，葡萄糖抑制 WaSTL1 的表达，而甘油使其去抑制/诱导表达，但表达水平更高。此外，还发现 LBCM1105 在有氧条件下利用甘油生长时会产生乙醇，这为细胞提供了一种电子还原逃逸途径，以补偿氰化物抗性呼吸（CRR）和甘油 3P 穿梭水平的氧化还原失衡。这项工作对于理解非酿酒酵母利用甘油的机制至关重要，考虑到它们在利用生物柴油副产物进行工业应用时的必要性。
