Fadool D A, Michel W C, Ache B W
Whitney Laboratory, University of Florida, St Augustine 32086.
J Exp Biol. 1993 Jan;174:215-33. doi: 10.1242/jeb.174.1.215.
Cultured lobster olfactory receptor neurons (ORNs) were surveyed for their odor sensitivity with whole-cell, voltage-clamp recording. The nature of the adequate stimuli, the degree of tuning (response spectra) of the cells, the threshold of sensitivity and the dual polarity of the odor-evoked currents are consistent with chemosensitivity in the cultured ORNs being olfactory. The ability of odors to evoke currents in cultured ORNs that lack processes suggests that lobster ORNs can be induced in vitro to insert all the elements of the transduction cascade in the soma, including those that might normally be confined to processes. This should greatly facilitate analysis of olfactory transduction in these cells.
利用全细胞膜片钳记录技术，对培养的龙虾嗅觉受体神经元（ORN）的气味敏感性进行了研究。适宜刺激的性质、细胞的调谐程度（反应谱）、敏感性阈值以及气味诱发电流的双极性，均表明培养的ORN中的化学敏感性具有嗅觉特性。气味能够在缺乏突起的培养ORN中诱发电流，这表明龙虾ORN在体外可被诱导在胞体中插入转导级联反应的所有元件，包括那些通常局限于突起中的元件。这将极大地促进对这些细胞嗅觉转导的分析。
