Shireman R B, Durieux J
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
Lipids. 1993 Feb;28(2):151-5. doi: 10.1007/BF02535780.
Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with the conventional spectrophotometric assays. The coefficient of variation for TC determinations was 4.5% or less and bias was 5% or less. The lipid micromethod assays are sensitive to 10 mg/dL or less, and the apolipoprotein assay to 1 mg/dL. Less than 100 microL of serum suffices for TC, TG and apoprotein assays; HDL-C requires an additional 100 microL of serum. Advantages of the micromethods include reductions in assay time and in the amount of reagents required.
微量滴定板法被开发用于血清总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和甘油三酯（TG）的酶法测定，以及载脂蛋白的比浊法测定。这些微量法得出的结果准确、精确，与传统分光光度法测定结果高度一致。TC测定的变异系数为4.5%或更低，偏差为5%或更低。脂质微量法测定对10mg/dL或更低浓度敏感，载脂蛋白测定对1mg/dL敏感。TC、TG和载脂蛋白测定所需血清量少于100微升；HDL-C测定还需要额外100微升血清。微量法的优点包括测定时间缩短和所需试剂量减少。
