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大基因组片段内编码的表达序列的磁珠捕获

Magnetic bead capture of expressed sequences encoded within large genomic segments.

作者信息

Tagle D A, Swaroop M, Lovett M, Collins F S

机构信息

Department of Human Genetics, University of Michigan Medical Center, Ann Arbor 48109-0650.

出版信息

Nature. 1993 Feb 25;361(6414):751-3. doi: 10.1038/361751a0.

DOI:10.1038/361751a0
PMID:8441473
Abstract

Magnetic bead capture utilizes biotin-streptavidin magnetic bead technology to isolate cDNAs rapidly from large genomic intervals, giving several thousand-fold enrichment of the selected cDNAs. The technique can allow parallel analysis of several large genomic segments of varying complexities and can be applied to the isolation of expressed sequences from various tissue sources.

摘要

磁珠捕获技术利用生物素-链霉亲和素磁珠技术从大的基因组区间快速分离cDNA，使所选cDNA富集数千倍。该技术可对几个复杂度不同的大基因组片段进行平行分析，并可用于从各种组织来源中分离表达序列。

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