Hemmerich P, von Mikecz A, Neumann F, Sözeri O, Wolff-Vorbeck G, Zoebelein R, Krawinkel U
Klinische Forschergruppe für Rheumatologie, Universitätsklinikum Freiburg, Germany.
Nucleic Acids Res. 1993 Jan 25;21(2):223-31. doi: 10.1093/nar/21.2.223.
By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should be a critical one as indicated by its high degree of structural conservation during evolution and its regulated expression in lymphoid cells. Human and rodent L7 proteins carry sequences similar to the basic-region-leucine-zipper(BZIP)-motif of DNA-binding eucaryotic transcription factors. We show here that the region of L7 carrying the latter motif mediates L7-dimerization and stable binding to DNA and RNA. A preferential binding to RNA-structures is demonstrated.
通过对由脂多糖（LPS）刺激的小鼠B淋巴细胞的mRNA构建的文库进行消减筛选，我们分离出了编码核糖体蛋白L7的cDNA克隆。人L7 mRNA是从活化的T淋巴细胞中克隆出来的。尽管目前尚不清楚L7在翻译装置中的具体功能，但鉴于其在进化过程中的高度结构保守性以及在淋巴细胞中的表达调控，它应该是一种关键蛋白。人和啮齿动物的L7蛋白携带的序列类似于DNA结合真核转录因子的碱性区域-亮氨酸拉链（BZIP）基序。我们在此表明，L7携带后一种基序的区域介导L7二聚化并与DNA和RNA稳定结合。还证明了它对RNA结构有优先结合作用。
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