Rocco R M, Abbott D C, Giese R W, Karger B L
Clin Chem. 1977;23(4):705-8.
A high-performance liquid chromatography method is presented for simultaneous analysis for procainamide and N-acetyl procainamide in plasma or serum. The procedure involves internal-standard addition, organic extraction, and separation on a reverse-phase column. The detection limit for procainamide is 0.1 mg/liter and the calibration plot is linear to at least 30 mg/liter. Comparison with a colorimetric assay for procainamide gave a correlation coefficient of 0.989. We checked for interference by a large series of appropriate drugs, and found none, nor did icteric or lipemic sera present problems.
本文介绍了一种高效液相色谱法，用于同时分析血浆或血清中的普鲁卡因胺和N - 乙酰普鲁卡因胺。该方法包括加入内标、有机萃取以及在反相柱上进行分离。普鲁卡因胺的检测限为0.1毫克/升，校准曲线在至少30毫克/升范围内呈线性。与普鲁卡因胺比色测定法的比较给出的相关系数为0.989。我们检查了大量相关药物的干扰情况，未发现干扰，黄疸或脂血血清也未造成问题。
