Dutcher J S, Strong J M
Clin Chem. 1977 Jul;23(7):1318-20.
We describe a routine method for determining concentrations of the antiarrhythmic drug procainamide and its active metabolite, N-acetylprocainamide, in plasma. A simple extraction of 1.0 ml of plasma is followed by separation and chromatographic analysis by use of a column containing microparticulate silica. p-nitro-N-(2-diethylaminoethyl)benzamide hydrochloride was synthesized and used as the internal standard. Total chromatographic time is only 7 min. The day-to-day CV during three months of daily use was less than 4% of the mean for each compound, and we saw no deterioration in column performance during this time. Phenobarbital, phenytoin, lidocaine, primidone, methsuximide, quinidine, and their metabolites do not interfere.
我们描述了一种用于测定血浆中抗心律失常药物普鲁卡因胺及其活性代谢物N - 乙酰普鲁卡因胺浓度的常规方法。先对1.0 ml血浆进行简单萃取，然后使用含有微粒硅胶的色谱柱进行分离和色谱分析。合成了对硝基 - N -（2 - 二乙氨基乙基）苯甲酰胺盐酸盐并用作内标。总色谱分析时间仅为7分钟。在日常使用的三个月期间，每种化合物的日间变异系数（CV）均小于平均值的4%，并且在此期间我们未观察到色谱柱性能下降。苯巴比妥、苯妥英、利多卡因、扑米酮、甲琥胺、奎尼丁及其代谢物均不产生干扰。
