Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.
作者信息
Spee J H, de Vos W M, Kuipers O P
机构信息
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
出版信息
Nucleic Acids Res. 1993 Feb 11;21(3):777-8. doi: 10.1093/nar/21.3.777.
Abstract
摘要
相似文献
1
Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.利用PCR和dITP的具有可调节突变频率的高效随机诱变方法。
Nucleic Acids Res. 1993 Feb 11;21(3):777-8. doi: 10.1093/nar/21.3.777.
2
Random mutagenesis by using mixtures of dNTP and dITP in PCR.
Methods Mol Biol. 1996;57:351-6. doi: 10.1385/0-89603-332-5:351.
3
Novel Random Mutagenesis Method for Directed Evolution.用于定向进化的新型随机诱变方法
Methods Mol Biol. 2017;1498:483-490. doi: 10.1007/978-1-4939-6472-7_32.
4
Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR.富含颠换的序列饱和诱变（SeSaM-Tv+）：一种具有连续核苷酸交换的随机诱变方法，可弥补易错PCR的偏差。
Biotechnol J. 2008 Jan;3(1):74-82. doi: 10.1002/biot.200700193.
5
[Region-specified PCR-mutagenesis: its application to locate epitopes for anti-RecA protein-monoclonal IgGs ].
Nihon Rinsho. 1994 Jun;52(6):1665-73.
6
Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.使用重水溶剂通过易错聚合酶链反应进行随机诱变。
Methods Mol Biol. 2017;1498:491-495. doi: 10.1007/978-1-4939-6472-7_33.
7
Development and Use of a Novel Random Mutagenesis Method: In Situ Error-Prone PCR (is-epPCR).一种新型随机诱变方法的开发与应用：原位易错PCR（is-epPCR）。
Methods Mol Biol. 2017;1498:497-506. doi: 10.1007/978-1-4939-6472-7_34.
8
An analysis of myo-[3H]inositol trisphosphates found in myo-[3H]inositol prelabelled avian erythrocytes.对在肌醇-[³H]预先标记的鸟类红细胞中发现的肌醇-[³H]三磷酸酯的分析。
Biochem J. 1989 Sep 15;262(3):727-37. doi: 10.1042/bj2620727.
9
Random mutagenesis by PCR.通过聚合酶链式反应进行随机诱变。
Curr Protoc Mol Biol. 2001 May;Chapter 8:Unit8.3. doi: 10.1002/0471142727.mb0803s51.
10
Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer.通过连接用突变引物扩增的两个PCR片段对较长插入片段进行诱变。
J Biosci Bioeng. 2009 Jan;107(1):95-7. doi: 10.1016/j.jbiosc.2008.09.003.
引用本文的文献
1
The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX.旧方法的力量：重新发现肌苷易错PCR以创建用于全细胞SELEX的起始文库。
Biosensors (Basel). 2025 Jul 12;15(7):448. doi: 10.3390/bios15070448.
2
An Extremely Sensitive Ultra-High Throughput Growth Selection Assay for the Identification of Amidase Activity.一种用于鉴定酰胺酶活性的超高灵敏度高通量生长选择测定法。
Appl Microbiol Biotechnol. 2024 Jun 24;108(1):392. doi: 10.1007/s00253-024-13233-z.
3
Conversion of the CG specific M.MpeI DNA methyltransferase into an enzyme predominantly methylating CCA and CCC sites.将 CG 特异性 M.MpeI DNA 甲基转移酶转化为主要甲基化 CCA 和 CCC 位点的酶。
Nucleic Acids Res. 2024 Feb 28;52(4):1896-1908. doi: 10.1093/nar/gkad1217.
4
Inosine Triphosphate Pyrophosphatase Dephosphorylates Ribavirin Triphosphate and Reduced Enzymatic Activity Potentiates Mutagenesis in Hepatitis C Virus.肌苷三磷酸焦磷酸酶使利巴韦林三磷酸去磷酸化，降低酶活性增强丙型肝炎病毒的突变。
J Virol. 2018 Sep 12;92(19). doi: 10.1128/JVI.01087-18. Print 2018 Oct 1.
5
High-resolution mapping of DNA polymerase fidelity using nucleotide imbalances and next-generation sequencing.利用核苷酸失衡和下一代测序技术对 DNA 聚合酶保真度进行高分辨率作图。
Nucleic Acids Res. 2018 Jul 27;46(13):e78. doi: 10.1093/nar/gky296.
6
MEK drives BRAF activation through allosteric control of KSR proteins.MEK 通过变构控制 KSR 蛋白驱动 BRAF 的激活。
Nature. 2018 Feb 22;554(7693):549-553. doi: 10.1038/nature25478. Epub 2018 Feb 12.
7
Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V.使用含脱氧肌苷的寡核苷酸和内切酶 V 定向克隆 DNA 片段。
BMC Biotechnol. 2013 Oct 4;13:81. doi: 10.1186/1472-6750-13-81.
8
Inosine triphosphate pyrophosphohydrolase (ITPA) polymorphic sequence variants in adult hematological malignancy patients and possible association with mitochondrial DNA defects.成人血液恶性肿瘤患者中肌苷三磷酸焦磷酸水解酶（ITPA）多态性序列变异体与线粒体 DNA 缺陷的可能相关性。
J Hematol Oncol. 2013 Mar 29;6:24. doi: 10.1186/1756-8722-6-24.
9
Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase from Thermotoga maritima.来自嗜热栖热菌的一种非典型核苷三磷酸焦磷酸酶的结构与功能表征
Acta Crystallogr D Biol Crystallogr. 2013 Feb;69(Pt 2):184-93. doi: 10.1107/S0907444912044630. Epub 2013 Jan 18.
10
Dislocation by the m-AAA protease increases the threshold hydrophobicity for retention of transmembrane helices in the inner membrane of yeast mitochondria.m-AAA 蛋白酶介导的易位增加了跨膜螺旋在酵母线粒体内膜中滞留的疏水性阈值。
J Biol Chem. 2013 Feb 15;288(7):4792-8. doi: 10.1074/jbc.M112.430892. Epub 2013 Jan 2.
本文引用的文献
1
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.使用嗜热栖热菌DNA聚合酶进行DNA测序以及对聚合酶链反应扩增的DNA进行直接测序。
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40. doi: 10.1073/pnas.85.24.9436.
2
Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.羊毛硫抗生素乳酸链球菌素Z（一种天然的乳酸链球菌素变体）的鉴定与特性分析。
Eur J Biochem. 1991 Nov 1;201(3):581-4. doi: 10.1111/j.1432-1033.1991.tb16317.x.
3
Improved site-directed mutagenesis method using PCR.利用聚合酶链式反应改进的定点诱变方法。
Nucleic Acids Res. 1991 Aug 25;19(16):4558. doi: 10.1093/nar/19.16.4558.
4
Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.利用Taq DNA聚合酶通过聚合酶链式反应对基因大小的DNA分子进行随机诱变。
Nucleic Acids Res. 1991 Nov 11;19(21):6052. doi: 10.1093/nar/19.21.6052.
Zotero 插件