Suppr超能文献

对在肌醇-[³H]预先标记的鸟类红细胞中发现的肌醇-[³H]三磷酸酯的分析。

An analysis of myo-[3H]inositol trisphosphates found in myo-[3H]inositol prelabelled avian erythrocytes.

作者信息

Stephens L R, Hawkins P T, Downes C P

机构信息

Smith Kline and French Research Limited, The Frythe, Welwyn, Herts., U.K.

出版信息

Biochem J. 1989 Sep 15;262(3):727-37. doi: 10.1042/bj2620727.

DOI:10.1042/bj2620727
PMID:2590163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133335/
Abstract

Evidence is presented to show that acid extracts of avian erythrocytes prelabelled for 24-48 h with myo-[3H]inositol contain the following myo-[3H]inositol trisphosphates (expressed as a percentage of total myo-[3H]inositol trisphosphates extracted): 36% myo-[3H]inositol 1,4,5-trisphosphate; 33.7% myo-[3H]inositol 1,3,4-trisphosphate; 13% myo-[3H]inositol 3,4,5-trisphosphate; 9.7% myo-[3H]inositol 3,4,6-trisphosphate; 4.4% myo-[3H]inositol 1,4,6-trisphosphate and 3.3% myo-[3H]inositol 1,3,6-trisphosphate. The only phosphatidyl-myo-[3H]inositol bisphosphate that could be detected in [3H]Ins-prelabelled avian erythrocytes was phosphatidyl-myo-[3H]inositol 4,5-bisphosphate. Cellular myo-[3H]inositol 3,4,5-trisphosphate may be synthesized by dephosphorylation of myo-[3H]inositol 3,4,5,6-tetrakisphosphate. D- and L-myo-[3H]inositol 1,4,6-trisphosphate and D- and L-myo-[3H]inositol 1,3,6-trisphosphate may be dephosphorylation products of myo-[3H]inositol 1,3,4,6-tetrakisphosphate.

摘要

有证据表明，用肌醇-[3H]预标记24 - 48小时的禽红细胞酸性提取物含有以下肌醇-[3H]三磷酸（以提取的总肌醇-[3H]三磷酸的百分比表示）：36%的肌醇-[3H]1,4,5 - 三磷酸；33.7%的肌醇-[3H]1,3,4 - 三磷酸；13%的肌醇-[3H]3,4,5 - 三磷酸；9.7%的肌醇-[3H]3,4,6 - 三磷酸；4.4%的肌醇-[3H]1,4,6 - 三磷酸和3.3%的肌醇-[3H]1,3,6 - 三磷酸。在[3H]肌醇预标记的禽红细胞中能够检测到的唯一磷脂酰 - 肌醇-[3H]二磷酸是磷脂酰 - 肌醇4,5 - 二磷酸。细胞内的肌醇-[3H]3,4,5 - 三磷酸可能是由肌醇-[3H]3,4,5,6 - 四磷酸去磷酸化合成的。D - 和L - 肌醇-[3H]1,4,6 - 三磷酸以及D - 和L - 肌醇-[3H]1,3,6 - 三磷酸可能是肌醇-[3H]1,3,4,6 - 四磷酸的去磷酸化产物。

相似文献

1
An analysis of myo-[3H]inositol trisphosphates found in myo-[3H]inositol prelabelled avian erythrocytes.对在肌醇-[³H]预先标记的鸟类红细胞中发现的肌醇-[³H]三磷酸酯的分析。
Biochem J. 1989 Sep 15;262(3):727-37. doi: 10.1042/bj2620727.
2
L-myo-inositol 1,4,5,6-tetrakisphosphate is present in both mammalian and avian cells.L-肌醇1,4,5,6-四磷酸存在于哺乳动物和鸟类细胞中。
Biochem J. 1988 Jan 1;249(1):271-82. doi: 10.1042/bj2490271.
3
Product-precursor relationships amongst inositol polyphosphates. Incorporation of [32P]Pi into myo-inositol 1,3,4,6-tetrakisphosphate, myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 3,4,5,6-tetrakisphosphate and myo-inositol 1,3,4,5,6-pentakisphosphate in intact avian erythrocytes.肌醇多磷酸之间的产物-前体关系。[32P]磷酸根离子掺入完整禽红细胞中的肌醇1,3,4,6-四磷酸、肌醇1,3,4,5-四磷酸、肌醇3,4,5,6-四磷酸和肌醇1,3,4,5,6-五磷酸。
Biochem J. 1990 Jan 15;265(2):435-52. doi: 10.1042/bj2650435.
4
Inositol trisphosphates in carbachol-stimulated rat parotid glands.卡巴胆碱刺激的大鼠腮腺中的肌醇三磷酸
Biochem J. 1984 Oct 1;223(1):237-43. doi: 10.1042/bj2230237.
5
Enantiomers of myo-inositol-1,3,4-trisphosphate and myo-inositol-1,4,6 -trisphosphate: stereospecific recognition by cerebellar and platelet myo-inositol-1,4,5-trisphosphate receptors.肌醇-1,3,4-三磷酸和肌醇-1,4,6-三磷酸的对映体：小脑和血小板肌醇-1,4,5-三磷酸受体的立体特异性识别
Mol Pharmacol. 1996 Nov;50(5):1223-30.
6
The Ca2+ release activities of D-myo-inositol 1,4,5-trisphosphate analogs are quantized.D-肌醇1,4,5-三磷酸类似物的Ca2+释放活性是量化的。
Cell Calcium. 1991 Jun;12(6):395-401. doi: 10.1016/0143-4160(91)90065-m.
7
Synthesis of D- and L-myo-inositol 2,4,5-trisphosphate and trisphosphorothioate: structural analogues of D-myo-inositol 1,4,5-trisphosphate.D-和L-肌醇2,4,5-三磷酸酯及三硫代磷酸酯的合成：D-肌醇1,4,5-三磷酸酯的结构类似物
Carbohydr Res. 2002 Nov 5;337(20):1795-801. doi: 10.1016/s0008-6215(02)00289-6.
8
Agonist-stimulated inositol phosphate metabolism in avian erythrocytes.激动剂刺激的禽类红细胞中的肌醇磷酸代谢。
Biochem J. 1990 Jul 1;269(1):65-72. doi: 10.1042/bj2690065.
9
Formation of [3H]inositol metabolites in rat hippocampal formation slices prelabelled with [3H]inositol and stimulated with carbachol.用[3H]肌醇预标记并经卡巴胆碱刺激的大鼠海马结构切片中[3H]肌醇代谢产物的形成。
J Neurochem. 1989 Mar;52(3):713-21. doi: 10.1111/j.1471-4159.1989.tb02513.x.
10
L-myo-inositol 1,4,5,6-tetrakisphosphate (3-hydroxy)kinase.L-肌醇1,4,5,6-四磷酸（3-羟基）激酶
Biochem J. 1988 Jan 1;249(1):283-92. doi: 10.1042/bj2490283.

引用本文的文献

1
Has Inositol Played Any Role in the Origin of Life?肌醇在生命起源中发挥过任何作用吗？
Life (Basel). 2017 Jun 5;7(2):24. doi: 10.3390/life7020024.
2
Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1.果蝇 S3 细胞中内源性细胞因子 (GBP)对 PLC 的激活及其作为研究通过 ITPK1 进行肌醇磷酸信号转导的模型的应用。
Biochem J. 2012 Dec 1;448(2):273-83. doi: 10.1042/BJ20120730.
3
Molecular characterization of an Arabidopsis gene encoding a phospholipid-specific inositol polyphosphate 5-phosphatase.一个编码磷脂特异性肌醇多磷酸5-磷酸酶的拟南芥基因的分子特征分析
Plant Physiol. 2004 Jun;135(2):938-46. doi: 10.1104/pp.104.040253. Epub 2004 Jun 4.
4
Metabolism and possible compartmentalization of inositol lipids in isolated rat-liver nuclei.大鼠离体肝细胞核中肌醇脂质的代谢及可能的区室化
Biochem J. 1997 Oct 15;327 ( Pt 2)(Pt 2):569-76. doi: 10.1042/bj3270569.
5
Metabolic evidence for PtdIns(4,5)P2-directed phospholipase C in permeabilized plant protoplasts.透化植物原生质体中磷脂酰肌醇 -4,5- 二磷酸（PtdIns(4,5)P2）导向的磷脂酶C的代谢证据
Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):123-31. doi: 10.1042/bj3240123.
6
Metabolic evidence for the order of addition of individual phosphate esters in the myo-inositol moiety of inositol hexakisphosphate in the duckweed Spirodela polyrhiza L.浮萍（Spirodela polyrhiza L.）中肌醇六磷酸肌醇部分单个磷酸酯添加顺序的代谢证据
Biochem J. 1996 Feb 15;314 ( Pt 1)(Pt 1):227-33. doi: 10.1042/bj3140227.
7
Inositol phosphates in the duckweed Spirodela polyrhiza L.浮萍（紫萍）中的肌醇磷酸
Biochem J. 1996 Feb 15;314 ( Pt 1)(Pt 1):215-25. doi: 10.1042/bj3140215.
8
Agonist-stimulated inositol phosphate metabolism in avian erythrocytes.激动剂刺激的禽类红细胞中的肌醇磷酸代谢。
Biochem J. 1990 Jul 1;269(1):65-72. doi: 10.1042/bj2690065.
9
Identification in extracts from AR4-2J cells of inositol 1,4,5-trisphosphate by its susceptibility to inositol 1,4,5-trisphosphate 3-kinase and 5-phosphatase.通过对肌醇1,4,5-三磷酸3-激酶和5-磷酸酶的敏感性在AR4-2J细胞提取物中鉴定肌醇1,4,5-三磷酸
Biochem J. 1990 Jul 1;269(1):195-200. doi: 10.1042/bj2690195.
10
Rat liver contains a potent endogenous inhibitor of inositol 1,3,4,5-tetrakisphosphate 3-phosphatase.大鼠肝脏含有一种有效的肌醇1,3,4,5-四磷酸3-磷酸酶内源性抑制剂。
Biochem J. 1990 May 1;267(3):831-4. doi: 10.1042/bj2670831.

本文引用的文献

1
A high-performance liquid chromatographic method to measure 32P incorporation into phosphorylated metabolites in cultured cells.一种用于测量32P掺入培养细胞中磷酸化代谢物的高效液相色谱法。
Anal Biochem. 1982 Aug;124(2):421-4. doi: 10.1016/0003-2697(82)90059-8.
2
Inositol trisphosphates in carbachol-stimulated rat parotid glands.卡巴胆碱刺激的大鼠腮腺中的肌醇三磷酸
Biochem J. 1984 Oct 1;223(1):237-43. doi: 10.1042/bj2230237.
3
A simple assay method for determination of the specific radioactivity of the gamma-phosphate group of 32P-labelled ATP.一种用于测定32P标记的ATP的γ-磷酸基团比放射性的简单测定方法。
Biochem J. 1983 Mar 15;210(3):717-20. doi: 10.1042/bj2100717.
4
The structure of triphosphoinositide from beef brain.来自牛脑的三磷酸肌醇的结构。
Biochim Biophys Acta. 1966 Dec 7;125(3):413-21. doi: 10.1016/0005-2760(66)90029-4.
5
The triphosphoinositide phosphomonoesterase of brain tissue.脑组织中的三磷酸肌醇磷酸单酯酶。
Biochem J. 1964 May;91(2):244-50. doi: 10.1042/bj0910244.
6
The triphosphoinositide phosphodiesterase of brain tissue.脑组织的三磷酸肌醇磷酸二酯酶
Biochem J. 1964 May;91(2):237-43. doi: 10.1042/bj0910237.
7
The hydrolysis of triphosphoinositide by extracts of ox brain.牛脑提取物对三磷酸肌醇的水解作用。
Biochem J. 1964 May;91(2):233-6. doi: 10.1042/bj0910233.
8
Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.毒蕈碱受体刺激大鼠大脑皮层切片后肌醇1,3,4,5-四磷酸的快速形成。
Biochem J. 1985 Nov 15;232(1):211-5. doi: 10.1042/bj2320211.
9
Inositol 1,2-cyclic 4,5-trisphosphate concentration relative to inositol 1,4,5-trisphosphate in pancreatic minilobules on stimulation with carbamylcholine in the absence of lithium. Possible role as a second messenger in long- but not short-term responses.在无锂情况下用氨甲酰胆碱刺激时，胰腺小叶中1,2-环肌醇4,5-三磷酸相对于肌醇1,4,5-三磷酸的浓度。在长期而非短期反应中作为第二信使的可能作用。
J Biol Chem. 1987 Oct 15;262(29):13892-5.
10
Metabolism of inositol 1,3,4-trisphosphate to a new tetrakisphosphate isomer in angiotensin-stimulated adrenal glomerulosa cells.在血管紧张素刺激的肾上腺球状带细胞中，肌醇1,3,4-三磷酸代谢为一种新的四磷酸异构体。
J Biol Chem. 1987 Jul 25;262(21):9952-5.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验