Kizaki H, Matsuo I, Sakurada T
Clin Chim Acta. 1977 Feb 15;75(1):1-4. doi: 10.1016/0009-8981(77)90492-2.
Xanthine oxidase and guanase activities in normal and psoriatic epidermis were demonstrated and accurately assayed by the new micro-assay methods which rely on the isolation of 14C-labelled end-products, xanthine and uric acid, from the substrates, hypoxanthine and guanine, by electrophoresis on cellulose acetate membrane using 0.1 M borate buffer, pH 9.0. The average specific activities of xanthine oxidase and guanase in normal epidermis were 10.2 and 55.0 pmol/min/mg protein, respectively, and in psoriatic epidermis, 52.1 and 980.6 pmol/min/mg protein, respectively. Increased activities of these enzymes in psoriatic epidermis suggested that urate formation might be enhanced in the psoriatic lesions resulting from an increased turn-over of nucleic acids.
采用新的微量测定方法，通过在pH 9.0的0.1 M硼酸盐缓冲液中于醋酸纤维素膜上进行电泳，从底物次黄嘌呤和鸟嘌呤中分离出14C标记的终产物黄嘌呤和尿酸，证实并精确测定了正常和银屑病表皮中的黄嘌呤氧化酶和鸟嘌呤酶活性。正常表皮中黄嘌呤氧化酶和鸟嘌呤酶的平均比活性分别为10.2和55.0 pmol/分钟/毫克蛋白，而在银屑病表皮中分别为52.1和980.6 pmol/分钟/毫克蛋白。银屑病表皮中这些酶活性的增加表明，由于核酸周转增加，银屑病皮损中尿酸盐的形成可能会增强。
