Kizaki H, Sakurada T
J Lab Clin Med. 1977 May;89(5):1135-44.
Xanthine oxidase, guanase, 5'-nucleotidase, and adenosine deaminase in human epidermis were demonstrated and accurately assayed with about 20 microng. of tissue by the new micro-assay methods which rely on the isolation of isotopically labeled end products from the substrates by electrophoresis on cellulose acetate membranes. These assay methods are rapid, reliable, and sensitive and are highly suitable for studies of small amount of human tissue. Theses methods for the separation of purine derivatives with cellulose acetate membrane will also permit the assays of purine nucleoside phosphorylase and nucleoside kinase.
利用新的微量测定方法，在约20微克人体表皮组织中证实并精确测定了黄嘌呤氧化酶、鸟嘌呤酶、5'-核苷酸酶和腺苷脱氨酶。这些新方法是通过在醋酸纤维素膜上进行电泳，从底物中分离出同位素标记的终产物。这些测定方法快速、可靠且灵敏，非常适合用于少量人体组织的研究。这些利用醋酸纤维素膜分离嘌呤衍生物的方法也可用于测定嘌呤核苷磷酸化酶和核苷激酶。
