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[整株植物胚囊和花粉复合体永久制片的快速制作方法]

[Accelerated production of permanent preparations of whole plant embryo sacs and pollen complexes].

作者信息

Solntseva M P, Levkoskiĭ V P

出版信息

Tsitol Genet. 1978 Nov-Dec;12(6):489-92.

PMID:84420
Abstract

Original methods are described for producing constant preparations of plant integral embryo sacs and pollen complexes isolated by means of tissue maceration with Helix pomatia gastric juice enzymes. The Feulgen stained preparations are put into the Canada balsam. According to this procedure there is no need of paraffin treatment and work with a microtome; it makes use of other methods which highly accelerate the production of constant preparations. In connection with putting the object in Canada balsam the method of treatment and the succession of operations are changed as compared to the routine procedure of producing total preparations of embryo sacks.

摘要

描述了通过用苹果蜗牛胃液酶进行组织浸软来制备植物完整胚囊和花粉复合体固定制剂的原始方法。将福尔根染色的制剂放入加拿大树胶中。按照这个程序,不需要进行石蜡处理和使用切片机;它采用了其他能大大加快固定制剂制备速度的方法。与将物体放入加拿大树胶相关,与制备胚囊整体制剂的常规程序相比,处理方法和操作顺序有所改变。

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