McKinnon K P, Madden M C, Noah T L, Devlin R B
TRC Environmental Corporation, Chapel Hill, North Carolina 27514.
Toxicol Appl Pharmacol. 1993 Feb;118(2):215-23. doi: 10.1006/taap.1993.1027.
Eicosanoids released after ozone exposure of a human bronchial epithelial cell line, BEAS-S6, were analyzed by high-pressure liquid chromatography (HPLC) of supernatants from exposed cells prelabeled with [3H]arachidonic acid. BEAS cells released thromboxane B2 (TxB2), prostaglandin E2 (PGE2), leukotriene C4 (LTC4), LTD4, LTE4, and 12-hydroxyheptadecatrienoic acid (HHT) after exposure to ozone at concentrations of 0.1, 0.25, 0.5, and 1.0 ppm. The eicosanoids were identified by coelution with authentic standards. The largest product from ozone-exposed BEAS cells was the most polar peak, designated Peak 1. Release of cyclooxygenase products such as TxB2, PGE2, and HHT was inhibited by acetylsalicylic acid. Peaks that migrated with authentic standards for LTB4, LTC4, and LTD4 were inhibited by the lipoxygenase inhibitor nordihydroguaiaretic acid. The leukotrienes LTB4 and LTC4/D4 could also be detected by immunoassay of concentrated peak fractions. Thus BEAS cells released eicosanoids from cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism following exposure to ozone. Airway epithelial cells may be an important source of eicosanoids following ozone stimulation in humans.
利用高压液相色谱法（HPLC）对经[3H]花生四烯酸预标记的人支气管上皮细胞系BEAS - S6暴露于臭氧后释放的类二十烷酸进行了分析。BEAS细胞在暴露于浓度为0.1、0.25、0.5和1.0 ppm的臭氧后，释放出血栓素B2（TxB2）、前列腺素E2（PGE2）、白三烯C4（LTC4）、LTD4、LTE4和12 - 羟基十七碳三烯酸（HHT）。通过与标准品共洗脱鉴定类二十烷酸。臭氧暴露的BEAS细胞产生的最大产物是最具极性的峰，命名为峰1。环氧化酶产物如TxB2、PGE2和HHT的释放受到乙酰水杨酸的抑制。与白三烯B4、LTC4和LTD4标准品迁移一致的峰受到脂氧合酶抑制剂去甲二氢愈创木酸的抑制。白三烯LTB4和LTC4/D4也可通过对浓缩峰馏分进行免疫测定来检测。因此，BEAS细胞在暴露于臭氧后从花生四烯酸代谢的环氧化酶和脂氧合酶途径释放类二十烷酸。在人类中，臭氧刺激后气道上皮细胞可能是类二十烷酸的重要来源。
