Lim H H, Buttery J E
Clin Chim Acta. 1977 Feb 15;75(1):9-12. doi: 10.1016/0009-8981(77)90494-6.
A simple, rapid and accurate colorimetric method for the determination of ethanol in serum was studied. Ethanol is converted to acetaldehyde by alcohol dehydrogenase, and the NADH formed transfers its hydrogen through the phenazine methosulphate-p-iodonitrotetrazolium violet (PMS-INT) system to produce the red formazan which is stable and absorbs at 505 nm. The method correlates very well with an enzymatic-UV method, r=0.98 (p less than 0.001). The precision (95% limits) of the method for samples ranging from 11 to 145 mg/dl was +/-6.6%, and the recovery averaged 99%.
研究了一种用于测定血清中乙醇的简单、快速且准确的比色法。乙醇通过乙醇脱氢酶转化为乙醛，生成的NADH通过吩嗪硫酸甲酯-对碘硝基四氮唑紫（PMS-INT）系统传递其氢，产生稳定的红色甲臜，并在505nm处有吸收。该方法与酶促紫外法相关性非常好，r = 0.98（p小于0.001）。对于浓度范围为11至145mg/dl的样品，该方法的精密度（95%置信限）为±6.6%，回收率平均为99%。
