Hellmén E, Bergström R, Holmberg L, Spångberg I B, Hansson K, Lindgren A
Department of Pathology, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala.
Vet Pathol. 1993 Jan;30(1):20-7. doi: 10.1177/030098589303000103.
The prognostic variables of 223 consecutively sampled spontaneous mammary tumors from female dogs were studied. These variables included flow cytometric DNA analysis and cell proliferation measured as cells in S-phase rate evaluated from DNA histograms. The dogs were surgically treated, in most cases with unilateral mastectomy (all mammary glands), and 202 of the 223 dogs were studied temporally following surgery. Univariate analysis with correction for age indicated that the variables of lymph node metastasis, elevated S-phase rate, presence of a sarcoma, DNA aneuploidy, and ulceration and infiltrative growth into underlying tissue had a statistically significant negative influence on the survival rates of dogs with a diagnosed malignant tumor. Similar results were obtained from tests on all dogs, but tumor size and its relative hazard increased with increasing size of the tumors, regardless of whether total or disease-specific mortality was considered. Using multivariate-analysis conducted Cox's proportional hazards model, elevated S-phase rate, increased age, and presence of a sarcoma remained statistically significant risk factors. The prognostic value of DNA ploidy and lymph node status varied depending on choice of end point. The study of tumor growth pattern and tumor size provided no prognostic information in the multivariate analysis. Flow cytometric cell analysis, including S-phase rate and DNA ploidy, is of value in predicting the prognosis of canine mammary tumors and can be used as a new prognostic tool to improve the preoperative diagnostics of canine mammary tumors.
对223例连续采样的雌性犬自发性乳腺肿瘤的预后变量进行了研究。这些变量包括流式细胞术DNA分析和细胞增殖，细胞增殖以从DNA直方图评估的S期细胞率来衡量。这些犬接受了手术治疗，大多数情况下进行单侧乳房切除术（所有乳腺），并且在223只犬中有202只在术后进行了随访研究。校正年龄后的单因素分析表明，淋巴结转移、S期率升高、肉瘤的存在、DNA非整倍体以及溃疡和向深层组织的浸润性生长等变量对诊断为恶性肿瘤的犬的生存率具有统计学上显著的负面影响。对所有犬进行的测试也得到了类似结果，但无论考虑的是总死亡率还是疾病特异性死亡率，肿瘤大小及其相对风险都随着肿瘤尺寸的增加而增加。使用Cox比例风险模型进行多因素分析，S期率升高、年龄增加和肉瘤的存在仍然是具有统计学意义的风险因素。DNA倍体和淋巴结状态的预后价值因终点的选择而异。在多因素分析中，肿瘤生长模式和肿瘤大小的研究未提供预后信息。流式细胞术细胞分析，包括S期率和DNA倍体，在预测犬乳腺肿瘤的预后方面具有价值，并且可以用作一种新的预后工具来改善犬乳腺肿瘤的术前诊断。
