Takahashi H, Utsuyama M, Kurashima C, Mori H, Hirokawa K
Department of Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
Acta Neuropathol. 1993;85(2):159-66. doi: 10.1007/BF00227763.
A monoclonal antibody (AmT-1) produced against synthetic amyloid beta peptide (1-28 residues) was revealed to be reactive with amyloid beta peptide blotted on nitrocellulose membrane, but not with that dissolved in sodium dodecyl sulfate and electrophoresed. AmT-1 immunostained senile plaques of typical, primitive and diffuse type, as well as amyloid deposits in cerebral vessels. It also reacted with neuronal and glial cells of normal and Alzheimer's disease (AD) brains. In addition, AmT-1 was also reactive strongly with lipofuscin pigments of adrenal reticular cells, and weakly with those of eccrine glands and liver cells. A rat neural cell line (PC12h) was reactive with AmT-1. By immunoelectron microscopy, a positive reaction was seen in ribosomes along the rough endoplasmic reticulum of nerve cells and PC12h cells. By immunoprecipitation, AmT1 reacted with a band at 36 kDa in the brain homogenates from Ad patients as well as from normal aged subjects. By immunoblotting analysis, AmT1 reacted with a band at 36 kDa in the cytosolic fraction of PC12 cells, and three bands (12-17 kDa) in the lipopigment fraction of the adrenal gland. These findings suggest that the cerebral amyloid deposits contain substance(s) having an epitope common to neuronal cells and lipofuscin pigments. The possible relationship between cerebral amyloid deposits and lipofuscin pigments in systemic organs is discussed.
一种针对合成β淀粉样肽（1 - 28个残基）产生的单克隆抗体（AmT - 1），被发现可与印迹在硝酸纤维素膜上的β淀粉样肽发生反应，但不与溶解在十二烷基硫酸钠中并经电泳处理的β淀粉样肽反应。AmT - 1免疫染色典型、原始和弥漫型老年斑以及脑血管中的淀粉样沉积物。它还与正常和阿尔茨海默病（AD）脑的神经元和神经胶质细胞发生反应。此外，AmT - 1还与肾上腺网状细胞的脂褐素色素强烈反应，与外分泌腺和肝细胞的脂褐素色素反应较弱。大鼠神经细胞系（PC12h）与AmT - 1发生反应。通过免疫电子显微镜观察，在神经细胞和PC12h细胞的粗面内质网沿线的核糖体中可见阳性反应。通过免疫沉淀，AmT1与AD患者以及正常老年受试者脑匀浆中36 kDa的条带发生反应。通过免疫印迹分析，AmT1与PC12细胞胞质部分中36 kDa的条带以及肾上腺脂褐素部分中的三条带（12 - 17 kDa）发生反应。这些发现表明，脑淀粉样沉积物含有与神经元细胞和脂褐素色素具有共同表位的物质。本文讨论了脑淀粉样沉积物与全身器官中脂褐素色素之间可能的关系。
