Cohen M L, Golde T E, Usiak M F, Younkin L H, Younkin S G
Division of Neuropathology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1227-31. doi: 10.1073/pnas.85.4.1227.
To determine which cells within the brain produce beta-amyloid mRNA and to assess expression of the beta-amyloid gene in Alzheimer disease, we analyzed brain tissue from Alzheimer and control patients by in situ hybridization. Our results demonstrate that beta-amyloid mRNA is produced by neurons in the nucleus basalis of Meynert and cerebral cortex and that nucleus basalis perikarya from Alzheimer patients consistently hybridize more beta-amyloid probe than those from controls. These observations support the hypothesis that increased expression of the beta-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease.
为了确定大脑中的哪些细胞产生β-淀粉样蛋白mRNA，并评估阿尔茨海默病中β-淀粉样蛋白基因的表达，我们通过原位杂交分析了阿尔茨海默病患者和对照患者的脑组织。我们的结果表明，β-淀粉样蛋白mRNA由Meynert基底核和大脑皮层中的神经元产生，并且来自阿尔茨海默病患者的基底核周体与对照患者相比，始终能杂交更多的β-淀粉样蛋白探针。这些观察结果支持了这样的假说，即β-淀粉样蛋白基因表达增加在阿尔茨海默病患者大脑中的淀粉样蛋白沉积中起重要作用。
