Yui S, Sasaki T, Miyazaki A, Horiuchi S, Yamazaki M
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Arterioscler Thromb. 1993 Mar;13(3):331-7. doi: 10.1161/01.atv.13.3.331.
We previously reported that cell membrane components and lipoproteins were able to induce the growth of murine peritoneal macrophages. The aim of the present study was to examine whether macrophage growth could also be induced by chemically modified lipoproteins, such as acetylated low density lipoprotein (acetyl-LDL) or oxidized LDL, ligands known to be endocytosed by the macrophage scavenger receptors. When murine peritoneal exudate macrophages were cultured in vitro with 25-100 micrograms/mL acetyl-LDL or oxidized LDL, significant growth was induced. On comparing the dose-response curves of these LDLs, a more potent effect was seen with oxidized LDL than acetyl-LDL, especially on resident macrophages. On the other hand, growth of these cells was not stimulated by native (unmodified) LDL or high density lipoprotein. These in vitro data revealed a new function of chemically modified LDLs as effective inducers of macrophage cell growth. This aspect may be physiologically relevant to the growth of macrophage foam cells in situ in the development of atherosclerosis.
我们之前报道过细胞膜成分和脂蛋白能够诱导小鼠腹腔巨噬细胞生长。本研究的目的是检测化学修饰的脂蛋白，如乙酰化低密度脂蛋白（乙酰-LDL）或氧化型LDL，是否也能诱导巨噬细胞生长，这些配体已知可被巨噬细胞清道夫受体内吞。当用25-100微克/毫升的乙酰-LDL或氧化型LDL体外培养小鼠腹腔渗出巨噬细胞时，可诱导显著生长。比较这些LDL的剂量反应曲线时，发现氧化型LDL比乙酰-LDL的作用更强，尤其是对驻留巨噬细胞。另一方面，天然（未修饰）LDL或高密度脂蛋白不会刺激这些细胞生长。这些体外数据揭示了化学修饰的LDL作为巨噬细胞生长有效诱导剂的新功能。这一方面可能在动脉粥样硬化发展过程中与原位巨噬细胞泡沫细胞的生长在生理上相关。
