Houlné G, Schantz M L, Meyer B, Pozueta-Romero J, Schantz R
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Curr Genet. 1994 Nov-Dec;26(5-6):524-7. doi: 10.1007/BF00309944.
We have isolated cDNA and genomic clones corresponding to a gene highly and specifically expressed at the late stage of fruit-ripening in bell-pepper. Antibodies raised against the expressed protein allowed determination of the cellular localization of the gene product. The encoded protein is present only in chromoplasts from fully-ripe red fruits, as shown by Western analysis and import experiments. The corresponding mRNA accumulates to high levels during ripening at orange and red stages, but is not detected in yellow varieties impaired in the synthesis of ketocarotenoids. Several lines of evidence indicate that the encoded protein is an oxido-reductase involved in the synthesis of capsanthin and capsorubin.
我们已经分离出与一个在甜椒果实成熟后期高度特异性表达的基因相对应的cDNA和基因组克隆。针对所表达蛋白质产生的抗体能够确定该基因产物的细胞定位。如蛋白质免疫印迹分析和导入实验所示，编码的蛋白质仅存在于完全成熟的红色果实的有色体中。相应的mRNA在果实成熟的橙色和红色阶段积累到高水平，但在酮类胡萝卜素合成受损的黄色品种中未检测到。几条证据表明，编码的蛋白质是一种参与辣椒红素和辣椒玉红素合成的氧化还原酶。
